| 牛奶中硫氰酸根的检测方法优化 |
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| 引用本文： | 刘洋,葛怀礼,于和水.牛奶中硫氰酸根的检测方法优化[J].中国动物保健,2014(4):29-30,32. |
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| 作者姓名： | 刘洋 葛怀礼 于和水 |
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| 作者单位： | 唐山市畜牧水产品质量监测中心,河北唐山063700 |
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| 摘 要： | 建立离子色谱法检测牛奶中硫氰酸根的检测方法，解决了杂峰对样品峰的干扰。样品经乙腈沉淀蛋白，冷冻离心，取上清液过RP柱待测。优化了色谱条件，以5％的丙酮和5．0mmol／L的碳酸钠为缓冲液，保留时间为12．5min，回收率在94．51％-104．21％之间，RSD为1．95％。定量范围为0．1～2．0μg／mL，检出限为0．02μg／mL，本方法简单快速，重现性好，灵敏度、准确度高，适用于牛奶中硫氰酸根的检测
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| 关 键 词： | 离子色谱 牛奶 硫氰酸根 |
Optimization of Detection Method of Thiocyanate in Milk |
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| Liu Yang,Ge Huaili,Yu Heshui.Optimization of Detection Method of Thiocyanate in Milk[J].China Animal Health,2014(4):29-30,32. |
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| Authors: | Liu Yang Ge Huaili Yu Heshui |
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| Institution: | ( Tangshan Livestock and Aquatic Products Quality Monitoring Center, Tangshan, Hebei, 063200 ) |
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| Abstract: | Detection method of thiocyanate by ion chromatography was established in milk, solve the interference peak to peak. Samples were first deproteined with acetonitrile, deproteinized by high-speed frozen centrifugation, purified with the RP column. Under the optimal conditions, with 5% of acetone and 5.0 mmol/L of sodium carbon- ate as the running buffer. Retention time was 12.5 min. The recoveries ranged from 94.51% to 104.21%, and av- erage relative standard deviation was 1.95%. The liner range is 0.1- 2.0μg/mL with the detection limit of 0.02 μg/mL. The method is simple and rapid, reproducible, sensitive, accurate, suitable for the detection of thiocyanate in milk. |
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| Keywords: | ion chromatography milk thiocyanate |
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