| 猪圆环病毒2型LAMP检测方法的建立 |
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| 引用本文： | 陈星瑶,;张子群,;王晓龙.猪圆环病毒2型LAMP检测方法的建立[J].中国动物保健,2014(9):14-17. |
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| 作者姓名： | 陈星瑶 ;张子群 ;王晓龙 |
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| 作者单位： | [1]东北林业大学保护医学与生态安全研究中心,黑龙江哈尔滨150040; [2]黑龙江省出入境检验检疫局,黑龙江哈尔滨150000 |
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| 基金项目： | 国家公益性行业（质检）专项（201210018-2）资助. |
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| 摘 要： | 摘要：猪圆环病毒2型属于圆环病毒科圆环病毒属，是引起仔猪断奶后多系统衰竭综合征（PMws）的主要病原。该病在世界范围内广泛流行，给我国养猪业造成一定的经济损失。目前针对PCV-2的检测技术还有提升的空间，因此本研究针对PCV-2Cap基因高度保守的区域作为靶序列设计引物，建立了一种可快速、灵敏、特异地检测PCV-2的LAMP检测方法。
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| 关 键 词： | 猪圆环病毒2型 环介导等温扩增技术(LAMP) |
Establishment of Loop-mediated Isothermal Amplifi-cation for Detection of Porcine Circovirus Type 2 |
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| Institution: | Chen Xingyao, Zhang Ziqun, Wang Xiaolong(1 Center of Conservation Medicine ＆ Ecological Safety, Northeast Forestry University, Harbin, Heilongjiang, 150040; 2.Heilongjiang Entry-exit Inspection and Quarantine Bureau, Harbin, Heilongjiang, 150000) |
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| Abstract: | Porcine circovirus virus type 2, a member of the Circoviridae family, is the main pathogen in causing the Postweaning muhisystemic wasting syndrome （PMWS）.This disease has been becoming a world wide epidemic, which also raises economic loss in the pig-breeding industry in our country. There is room for improvement in PCV-2 inspecting. We designed primers according to highly conserved regions of Cap gene of PCV-2, and con- structed a quick, sensitive and specific detection method of LAMP for PCV-2. |
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| Keywords: | porcine circovirus virus type 2 1 oop-mediated isothermal amplification（LAMP） |
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