| 人工异源六倍体小麦部分同源基因的表达变化 |
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| 引用本文： | 王延召,聂利红,孙其信,胡学安,魏良明,周波.人工异源六倍体小麦部分同源基因的表达变化[J].河南农业科学,2012,41(11):19-21. |
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| 作者姓名： | 王延召 聂利红 孙其信 胡学安 魏良明 周波 |
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| 作者单位： | 1. 河南省农业科学院,河南郑州,450002
2. 西北农林科技大学,陕西杨凌,712100 |
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| 摘 要： | 为了研究小麦从四倍体到六倍体进化过程中部分同源基因的表达变化,以人工异源六倍体小麦SCA/SQ及其亲本SCAUP和SQ523为材料,采用cDNA-SSCP方法分析了208个部分同源基因不同拷贝的表达情况。结果表明,有10个来自父本的拷贝在人工异源六倍体小麦中沉默,进一步分析了这些沉默表达的基因,发现其中有5个是由于在DNA序列上丢失,而另外5个可能是由于甲基化或者其他机制引起的。
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| 关 键 词： | 人工异源六倍体小麦 多倍化 基因表达变化 |
Expression of Homoelogous Gene in Synthetic Hexaploid Wheat |
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| WANG Yan-zhao
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NIE Li-hong
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SUN Qi-xin
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HU Xue-an
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WEI Liang-ming
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ZHOU Bo.Expression of Homoelogous Gene in Synthetic Hexaploid Wheat[J].Journal of Henan Agricultural Sciences,2012,41(11):19-21. |
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| Authors: | WANG Yan-zhao NIE Li-hong SUN Qi-xin HU Xue-an WEI Liang-ming ZHOU Bo |
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| Institution: | 1 (1.Henan Academy of Agricultural Sciences,Zhengzhou 450002,China; 2.Northwest A &F University,Yangling 712100,China) |
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| Abstract: | In order to understand the homoeologous gene changes during the evolution of hexaploid wheat from tetroploid one,the artificial synthesized heterogenous hexaploid wheat SCA/SQ and its parents SCAUP and SQ523 were taken as materials to study the homoelogous gene expression by means of cDNA-SSCPanalysis.Results showed that 10 gene copies from male parent SQ523 were silenced in synthetic hexaploid wheat,among which 5 copies were caused by the loss of corresponding DNA sequences,and other 5 ones were due to methylation or other mechaniisms according to the DNA-SSCP analysis. |
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| Keywords: | synthetic hexaploid wheat polyploidization gene expression changes |
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