| 鸭疫里默氏杆菌原生质体制备与再生的研究 |
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| 引用本文： | 王春平,韦强,崔言顺,鲍国连,刘燕,邵泽香.鸭疫里默氏杆菌原生质体制备与再生的研究[J].浙江农业学报,2009,21(4):0. |
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| 作者姓名： | 王春平 韦强 崔言顺 鲍国连 刘燕 邵泽香 |
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| 作者单位： | 山东农业大学动物科技学院，山东泰安271018； 浙江省农业科学院畜牧兽医研究所，浙江杭州310021 |
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| 基金项目： | 浙江省重点科技攻关项目，浙江省农科院创新能力提升工程项目 |
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| 摘 要： | 以耐药标记的鸭疫里默氏杆菌TXRAl(CAd ，Elf)为亲本菌株，在DNase I始终存在的条件下，采用Lysozyme-EDTA法制备原生质体，然后在高渗琼脂平板上再生。系统地优化了影响原生质体制备及再生的各种条件，摸索出鸭疫里默氏杆菌TXRA1以Lysozyme 0．1 g／L作用20 min后补加EDTA至终浓度为0．01 mol／L继续作用45 min为最佳试验条件，并成功获得了92％的原生质体制备率和38．77％的再生率。
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| 关 键 词： | 鸭疫里默氏杆菌 原生质体 制备 再生 |
Studies on protoplast formation and regeneration of Riemerella anatipestifer strain |
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| WANG Chun-ping,WEI Qiang,CUI Yan-shun,BAO Guo-lian,LIU Yan,SHAO Ze-xiang.Studies on protoplast formation and regeneration of Riemerella anatipestifer strain[J].Acta Agriculturae Zhejiangensis,2009,21(4):0. |
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| Authors: | WANG Chun-ping WEI Qiang CUI Yan-shun BAO Guo-lian LIU Yan SHAO Ze-xiang |
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| Institution: | 1 College of Animal Science and Veterinary Medicine of Shandong Agricultrual University，Tai'an Shandong 271018，China；2 Institute of Animal Husbandry and Veterinary Science，Zhejiang Academy of Agricultural Sciences，Hangzhou Zhejiang 310021，China |
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| Abstract: | Using Riemerella anatipestifer TXRA1(Chl r，Ery s)strain with drug resistance label as the parental strain，protoplasts were pfe with lysozyme and EDTA at the presence of DNase I，then the protoplasts were regenerated on hypertonicmedium．Conditions of protoplast formation and regeneration were optimized．indicating that the optimum Riemerella anatipestifer TXRA1(Chl r，Ery s)added with 0．1 g／L lysozyme and reacted for 20 min and then EDTA Was added slowly to a final concentration of O．O1 mol/L and reacted for another 45 min, in which the protoplast formation rate was92％ and the regeneration rate was 38．77％ ． |
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| Keywords: | Riemerella anatipestifer protoplast formation regeneration |
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