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| 利用SSR分子标记进行棉花品种鉴定的研究 | |
| 引用本文： | 张小娟,陆徐忠,何团结,路曦结,郑曙峰,倪金龙,张冰清,杨剑波.利用SSR分子标记进行棉花品种鉴定的研究[J].棉花学报,2014,26(6):483-491. |
| 作者姓名： | 张小娟 陆徐忠 何团结 路曦结 郑曙峰 倪金龙 张冰清 杨剑波 |
| 作者单位： | 1. 安徽农业大学,合肥230031;安徽省农科院棉花研究所,安庆246003 2. 安徽省农科院水稻研究所,合肥,230031 3. 安徽省农科院棉花研究所,安庆,246003 4. 安徽农业大学,合肥,230031 |
| 基金项目： | 农业部农业行业标准研制计划 |
| 摘 要： | 利用均匀分布于棉花26条染色体上的39对SSR核心引物对16个不同来源的棉花品种样品进行区别与鉴定实验,探讨了棉花品种鉴定的取样方案和位点异同判读方法。通过比较不同取样量样品包含的遗传信息,确定了基本的取样量,并将样品间位点异同类型分为相同位点、疑似相同位点和差异位点。据此对16个品种进行位点异同性分析,结果表明同一品种的不同样品间差异位点数均在0~2间,而不同品种间的差异位点数介于5~25。结合品种间差异位点的阈值分析,研究认为当两样品间差异位点数2时,即判定为不同品种;当差异位点数≤2时,即判定为近似或极近似品种。 |
| 关 键 词： | 棉花 品种鉴定 SSR分析 带型差异 |
| 收稿时间： | 2014-03-17 |
Identification of Cotton Varieties Using SSR Molecular Markers |
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| ZHANG Xiao-Juan,LU Xu-Zhong,HE Tuan-Jie,LU Xi-Jie,ZHENG Shu-Feng,NI Jin-Long,ZHANG Bing-Qing,YANG Jian-Bo.Identification of Cotton Varieties Using SSR Molecular Markers[J].Cotton Science,2014,26(6):483-491. | |
| Authors: | ZHANG Xiao-Juan LU Xu-Zhong HE Tuan-Jie LU Xi-Jie ZHENG Shu-Feng NI Jin-Long ZHANG Bing-Qing YANG Jian-Bo |
| Institution: | 1. Anhui Agricultural University, Hefei 230031, China; 2. Institute of Cotton Research, Anhui Academy of Agricultural Sciences, Anqing, 246003, China; 3. Institute of Rice Research, Anhui Academy of Agricultural Sciences, Hefei 230031, China |
| Abstract: | To assess the sampling mode and site determination methods for identifying cotton varieties, 39 pairs of SSR primers distributed evenly on the 26 chromosomes of cotton were screened in 16 cotton varieties, and showed high polymorphism and good repeatability. The basic sampling amount was determined by comparing the genetic information of different sampling modes. The sites were classified into three types: the same locus, the suspected same locus, and the discrepant locus, according to the similarities and differences of sites. The similarities and differences of sites in the 16 varieties were analyzed using the above scheme. The results showed that the number of discrepant loci for the same variety was between 0 and 2, and the number for different varieties ranged from 5 to 25. Based on the analysis of the discrepant locus threshold between different varieties, samples can be determined to be different when the number of discrepant loci is more than 2, and similar when there are fewer than 2 discrepant loci. |
| Keywords: | cotton variety identification SSR analysis discrepant patterns |
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