| 月季RcLEA基因表达提高大肠杆菌对非生物胁迫耐性 |
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| 引用本文： | 蒋昌华.月季RcLEA基因表达提高大肠杆菌对非生物胁迫耐性[J].农业科学与技术,2010,11(6):79-82. |
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| 作者姓名： | 蒋昌华 |
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| 作者单位： | 上海植物园,上海,200231 |
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| 基金项目： | Key Scientific and Technological Project for Developing Agriculture from Shanghai Municipal Agriculture Commission (200810-4).上海市农委科技兴农重点攻关项目(沪农科攻字 |
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| 摘 要： | 目的]研究RcLEA基因在月季品种中热诱导表达模式及RcLEA基因对多种非生物胁迫耐性。方法]将耐热和不耐热月季品种进行38℃/3h热激处理,研究RCLEA基因在月季品种中的热诱导。表达模式;为验证月季RcLEA基因功能,将其转化E.coliBL21,将重组菌株BL21分别置于4℃、50℃及LiCl、NaCl、Na2CO3、CdCl2、H2O2胁迫下,研究重组菌株对高温、低温及非生物胁迫的响应。结果]38℃/3h热激处理后,该基因在耐热月季品种‘曼海姆宫殿’（Schlossmannieim,SM）、‘赌城’（Lasvegas,LV）中强表达,而在不耐热品种‘新十全’（Kordes＇Perfecta,KP）弱表达或不表达,表明该基因与月季耐热性关系密切。重组菌株提高了寄主大肠杆菌对高温、低温、重金属、高盐、高pH值、氧化等非生物胁迫的耐性,表明RcLEA参与了上述非生物胁迫的响应。结论]该研究为后续该基因导入不耐热月季品种提高月季耐热品质及其机理研究提供了思路,也为月季等园林观赏植物的耐热品种筛选提供了理论支持。
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| 关 键 词： | 月季 RcLEA基因 诱导表达 非生物胁迫 |
The Expression of RcLEA Gene Improves Tolerance of E. coli Cells to Abiotic Stress |
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| JIANG Chang-hua.The Expression of RcLEA Gene Improves Tolerance of E. coli Cells to Abiotic Stress[J].Agricultural Science ＆ Technology,2010,11(6):79-82. |
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| Authors: | JIANG Chang-hua |
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| Institution: | JIANG Chang-hua Shanghai Botanical Garden,Shanghai 200231 |
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| Abstract: | Objective] This study was to reveal the heat induced expression model of RcLEA gene and its tolerance to various abiotic stresses.Method] Heat resistant and heat sensitive varieties of Rosa hybrida L.were subjected to heat shock treatment at 38 ℃ for 3 h;then RcLEA gene from both varieties treated was cloned and transformed into Escherichia coli strain BL21;finally recombinant colonies were separately cultured at 4 ℃ and 50 ℃ under the stresses of LiCl,NaCl,Na2CO3,CdCl2 and H2O2 to study the responses of recombinant E.coli strains to high temperature,low temperature and some other abiotic stresses.Result] After heat shock treatment at 38 ℃ for 3 h,RcLEA gene expressed highly in ＇Schloss mannieim＇（SM）and ＇Las vegas＇（LV）variety,but weakly or even not expressed in ＇Kordes＇ Perfecta＇（KP）,indicating that this gene is closely related with heat resistance of R.hybrida.Compared with WT strains,recombinant clones showed higher tolerance to abiotic stresses including high temperature,low temperature,heavy metal,high salt,high pH value and oxidation,suggesting that RcLEA is concerned with the response of R.hybrida to abiotic stresses mentioned above.Conclusion] These results provide thoughts for increasing heat resistance by introducing RcLEA into heat sensitive R.hybrida varieties and studying the heat-resistant mechanism of R.hybrida,and also provide theoretical support for selecting heat resistant variety of landscape and ornamental plants like R.hybrida. |
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| Keywords: | RcLEA Rosa hybrida L RcLEA Induced expression Abiotic stress |
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