Response of the soil fungal community to multi-factor environmental changes in a temperate forest |
| |
| Institution: | 1. Centre of Oceanography of the Faculty of Sciences of the University of Lisbon (CO), Campo Grande, Lisbon 1749-016, Portugal;2. Physics Department & Centre for Environmental and Marine Studies (CESAM), University of Aveiro, Campus de Santiago, Aveiro 3810-193, Portugal;3. Animal Biology Department, Faculty of Sciences of the University of Lisbon (CO), Campo Grande, Lisbon 1749-016, Portugal;4. Biology Department & Centre for Environmental and Marine Studies (CESAM), University of Aveiro, Campus de Santiago, Aveiro 3810-193, Portugal;5. Institute of Marine Research–Marine and Environment Research Centre (IMAR-CMA), c/o Department of Zoology, Faculty of Sciences and Technology, University of Coimbra, Coimbra 3000, Portugal;1. Institute of Ecological Science, Department of Systems Ecology, Faculty of Earth and Life Sciences, VU University Amsterdam, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands;2. Institute of Ecological Science, Department of Animal Ecology, Faculty of Earth and Life Sciences, VU University Amsterdam, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands;3. Climate Impacts Research Centre, Department of Ecology and Environmental Science, Umeå University, 901 87 Umeå, Sweden;1. Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, T6G 2P5 Alberta, Canada;2. Department of Renewable Resources, University of Alberta, Edmonton, T6G 2H1 Alberta, Canada;3. Environmental Studies Program, Saint Michael’s College, Colchester, 05439 VT, USA;4. Range Resource Program, Alberta Environment and Parks, Edmonton, T5K 2M4 Alberta, Canada;1. State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;2. State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China |
| |
| Abstract: | Both environmental and climatic changes are known to influence soil microbial biomes in terrestrial ecosystems. However, there are limited data defining the interactive effects of multi-factor environmental disturbances, including N-deposition, precipitation, and air temperature, on soil fungal communities in temperate forests. A 3-year outdoor pot experiment was conducted to examine the temporal shifts of soil fungal communities in a temperate forest following N-addition, precipitation and air temperature changes. The shifts in the structure and composition of soil fungal communities were characterized by denaturing gradient gel electrophoresis and DNA sequencing. N-addition regimen induced significant alterations in the composition of soil fungal communities, and this effect was different at both higher and lower altitudes. The response of the soil fungal community to N-addition was much stronger in precipitation-reduced soils compared to soils experiencing enhanced precipitation. The combined treatment of N-addition and reduced precipitation caused more pronounced changes in the lower altitude versus those in the higher one. Certain fungal species in the subphylum Pezizomycotina and Saccharomycotina distinctively responded to N fertilization and soil water control at both altitudes. Redundancy discrimination analysis showed that changes in environmental factors and soil physicochemical properties explained 43.7% of the total variability in the soil fungal community at this forest ecosystem. Variations in the soil fungal community were significantly related to the altitude, soil temperature, total soil N content (TN) and pH value (P < 0.05). We present evidence for the interactive effects of N-addition, water manipulation and air temperature to reshape soil fungal communities in the temperate forest. Our data could provide new insights into predicting the response of soil micro-ecosystem to climatic changes. |
| |
| Keywords: | Fungal community Nitrogen fertilization Soil water control Altitude Denaturing gradient gel electrophoresis DNA sequencing |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
