| 绵羊BMPR-IB基因在分子标记育种中的应用效果 |
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| 引用本文： | 方翟,吴建平,徐建峰,罗艳茹,张利平,王磊,杨联,雷赵民.绵羊BMPR-IB基因在分子标记育种中的应用效果[J].新疆农业科学,2010,47(1):189-194. |
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| 作者姓名： | 方翟 吴建平 徐建峰 罗艳茹 张利平 王磊 杨联 雷赵民 |
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| 作者单位： | 甘肃农业大学动物科学技术学院,甘肃兰州,730070;中国农业大学动物科学技术学院,北京,100194 |
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| 基金项目： | 甘肃省农业生物技术研究与应用开发项目 |
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| 摘 要： | 目的]分析小尾寒羊、德国美利奴、德国美利奴(♂)和小尾寒羊(♀)杂交一代(简称DF1)共204 只个体的单核苷酸多态性及其与产羔数间的关系.方法]以绵羊BMPR-IB 基因作为候选基因,采用PCR-sscp 检测方法.结果]B等位基因在小尾寒羊、德国美利奴、德寒杂交一代中的频率分别为0.660、0.532和0.600,基因频率及基因型频率在3个群体间的分布差异不显著(P>0.05).BB、B+、++型的产羔数分别为2.86、2.27和2.08,BB 型显著高于B+ 和++ 型(P>0.05),B+ 型显著高于++ 型(P>0.05).结论]在以小尾寒羊为母本的杂交育种过程中,B等位基因是有效的辅助标记基因.
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| 关 键 词： | 绵羊 BMPR-IB 产羔数 相关性 |
| 收稿时间： | 2010-01-25 |
Research on Application Effect in Breeding of Molecular Marker in Sheep BMPR-IB Gene |
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| FANG Di,WU Jian-ping,XU Jian-feng,LUO Yan-ru,ZHANG Li-ping,WANG Lei,YANG Lian,LEI Zhao-min.Research on Application Effect in Breeding of Molecular Marker in Sheep BMPR-IB Gene[J].Xinjiang Agricultural Sciences,2010,47(1):189-194. |
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| Authors: | FANG Di WU Jian-ping XU Jian-feng LUO Yan-ru ZHANG Li-ping WANG Lei YANG Lian LEI Zhao-min |
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| Abstract: | Objective and Method]The polymorphism of bone morphogenetic protein receptor type IB gene (BMPR-IB gene) of 204 sheep of three breeds or strains ,which included Small Tail Han sheep,F_1 generation from German Merino(♂)×Small Tail Han sheep(♀) cross generation （DF1）, German Merino ,was analyzed by PCR-sscp. The relationship between genotype and litter size was also identified.Result and Conclusion]The results showed that the frequency of B allele in Small-Tail Han sheep, German Merino and DF1 was 0.660,0.532 and 0.600，there was no significant difference of distribution of gene frequency and genotype frequency in 3 groups.The litter size of BB,B+ and ++ was 2.86,2.27 and 2.08，BB were both significantly higher than B+ and ++ (P＜0.05),and B+ were significantly higher than ++ type. |
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| Keywords: | BMPR-IB sheep BMPR-IB litter size relationship |
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