| 拟南芥根部芥子酶活性测定条件及其与重组蛋白酶学活性比较研究 |
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| 引用本文： | 吴丽娟,马帅,付莉莉,孙雪飘,张家明.拟南芥根部芥子酶活性测定条件及其与重组蛋白酶学活性比较研究[J].中国农学通报,2012,28(27):183-188. |
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| 作者姓名： | 吴丽娟 马帅 付莉莉 孙雪飘 张家明 |
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| 作者单位： | 1. 中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室,海口571101;海南大学农学院,海口571101
2. 中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室,海口,571101 |
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| 基金项目： | 国家自然科学基金资助项目“拟南芥芥子酶‘假基因’TGG6活性等位基因的功能研究”（31070271） |
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| 摘 要： | 芥子酶是一类降解硫代葡萄糖苷的酶，该基因表达具有组织特异性。研究表明，新型芥子酶TGG4和TGG5都是在根部表达。为了研究TGG4、TGG5的功能作用，提取了生态型拟南芥Col-0根部以及含有重组蛋白酵母的芥子酶，进行了酶活性条件测定以及对比分析。结果表明：根部芥子酶有更高的抗坏血酸激活浓度为2 mmol/L；根部芥子酶同重组蛋白的最佳反应温度以及pH范围一致，在37℃，体系缓冲液pH 4.5时，根部芥子酶活性测定效果最好；NaCl对根部芥子酶和重组蛋白芥子酶都有强烈抑制作用。因此推断根部芥子酶在耐盐碱、耐高温方面没有起作用，该酶也不能帮助根部抵御病虫侵袭，但是对根部的生长起到重要作用。
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| 关 键 词： | 有机氮 有机氮 |
| 收稿时间： | 2011/12/26 0:00:00 |
| 修稿时间： | 3/1/2012 12:00:00 AM |
Determination of Myrosinase Assay Conditions in Arabidopsis thaliana Root and Its Comparition with Recombinant Protein Activities |
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| Wu Lijuan
,
Ma Shuai
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Fu Lili
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Sun Xuepiao
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Zhang Jiaming.Determination of Myrosinase Assay Conditions in Arabidopsis thaliana Root and Its Comparition with Recombinant Protein Activities[J].Chinese Agricultural Science Bulletin,2012,28(27):183-188. |
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| Authors: | Wu Lijuan Ma Shuai Fu Lili Sun Xuepiao Zhang Jiaming |
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| Institution: | 1Institute of Tropical Biotechnology Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou 571101; 2College of Agriculture, Hainan University, Haikou 571101) |
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| Abstract: | Glucosinolate-myrosinase is a kind of enzymes that degrade glycosidase, the express of this gene is tissue-specific. Research showed that, the new myrosinase TGG4 and TGG5 are found in Arabidopsis thaliana root. In order to find the function of TGG4 and TGG5, we extracted the myrosinase from ecological Col-0 root and the yeast contained recombinant protein, determined the enzyme activity condition and analyzed the different. It suggested that, the root myrosinase need higher ascorbic acid active concentration, it was about 2 mmol/L, the best reaction temperature and pH range were the same, for root myrosinase, the temperature was 37℃, and the pH was 4.5, for all myrosinase the NaCl had a strong inhibition of effect. The author believed that, root myrosinase did not work in salinity and themostability, and the enzyme also could not help root against disease and insect attack, but it might be play an important role in root growth. |
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| Keywords: | Arabidopsis thaliana myrosinase activity comparison |
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