| 不同基因型对花生胚小叶植株再生的影响 |
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| 引用本文： | 隋炯明,乔利仙,赵明霞,徐丽娟,王晶珊.不同基因型对花生胚小叶植株再生的影响[J].中国农学通报,2012,28(27):45-48. |
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| 作者姓名： | 隋炯明 乔利仙 赵明霞 徐丽娟 王晶珊 |
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| 作者单位： | 青岛农业大学生命科学学院/山东省高校植物生物技术重点实验室,山东青岛,266109 |
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| 基金项目： | 国家自然基金青年基金“花生几丁质酶基因、β-1 3-葡聚糖酶基因启动子的克隆与功能分析”（31101178）; 山东省自然基金“花生离体诱变及创造抗旱耐盐种质资源研究”（ZR2012CM014）;山东省自然基金青年基金“花生维生素E合成关键基因的克隆及其抗氧化机制研究”（ZR2011CQ026）; 山东省中青年科学家奖励基金“花生Chi基因及Glu基因的克隆、双价表达载体的构建及遗传转化研究”（BS2009NY028） |
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| 摘 要： | 以不同类型18个花生品种成熟种子的胚小叶为外植体，对不定芽诱导及植株再生进行了研究，旨在为花生遗传转化和离体诱变等提供培养方法。将胚小叶外植体培养在添加1 mg/L NAA和6 mg/L BAP的诱导培养基上，4周后转移到添加4 mg/L BAP的培养基上进行培养。结果表明，所有的供试品种芽点诱导率均高于60%，但5大类型间及品种间(62.2%~95.5%)存在显著差异，龙生型平均诱导率最高(87.2%)，其次是珍珠豆型(81.5%)，多粒型最低(63.1%)。将形成芽点的外植体转移到添加4 mg/L BAP的培养基上后，部分外植体从芽点上分化出不定芽，继续培养不定芽伸长并再生植株。植株再生率也存在显著性差异，最高的是珍珠豆型(83.5%~97.1%)，最低是多粒型(14.8%~22.0%)。
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| 关 键 词： | 淋溶模拟 淋溶模拟 |
| 收稿时间： | 2012/4/26 0:00:00 |
| 修稿时间： | 2012/6/18 0:00:00 |
Effect of Genotypes on Plant Regeneration of Embryonic Leaflet in Peanut |
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| Sui Jiongming
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Qiao Lixian
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Zhao Mingxia
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Xu Lijuan
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Wang Jingshan.Effect of Genotypes on Plant Regeneration of Embryonic Leaflet in Peanut[J].Chinese Agricultural Science Bulletin,2012,28(27):45-48. |
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| Authors: | Sui Jiongming Qiao Lixian Zhao Mingxia Xu Lijuan Wang Jingshan |
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| Institution: | (College of Life Science, Qingdao Agricultural University/ Key Lab of Plant Biotechnology in Universities of Shandong, Qingdao Shandong 266109) |
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| Abstract: | To provide culture method for genetic transformation and tissue culture in vitro, efficient adventitious shoot induction and plant regeneration of embryonic leaflets among 18 peanut cultivars in 5 types were studied in this paper. The embryonic leaflets were cultured on MSB5 induction medium （Murashige and Skoog salts plus B5 vitamins） supplemented with 1 mg/L NAA and 6 mg/L BAP, and then transferred to the medium supplemented with 4 mg/L BAP. The result showed that the frequencies of bud point induction were different significantly in the cultivars tested that ranged 62.2%-95.5% as well as in 5 peanut types. The highest induction frequency was obtained in Arachis hypogaea var. hirsuta （87.2% ）, followed by Arachis hypogaea var. rulgaris （81.5%）, the lowest was Arachis hypogaea var. fastigiata （63.1%）. When the explants with bud points were transferred to the medium supplemented with 4 mg/L BAP, some of the bud points developed and then regenerated plants. The regeneration rate of plant was also different, the cultivars in Arachis hypogaea var. rulgaris produced higher frequencies （83.5%-97.1%）, while that of Arachis hypogaea var. fastigiata produced lower frequencies （14.8%-22.0%）. |
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| Keywords: | peanut （Arachis hypogaea L ） embryonic leaflet tissue culture adventitious shoot induction plant regeneration |
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