| 猪耳缘成纤维细胞的体外培养 |
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| 引用本文： | 薄旭,林浩,刘云海,郭勇,倪和民.猪耳缘成纤维细胞的体外培养[J].中国农学通报,2012,28(23):32-35. |
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| 作者姓名： | 薄旭 林浩 刘云海 郭勇 倪和民 |
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| 作者单位： | 北京农学院动物科学技术学院,北京,102206 |
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| 基金项目： | 国家自然基金面上资助项目“不同来源早期绵羊胚胎IFNtau差异表达及其体外诱导子宫上皮细胞calectin15表达的研究”（31072185） |
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| 摘 要： | 为了建立猪耳缘成纤维细胞培养体系，为猪核移植实验做准备。主要采用组织块法进行猪耳缘成纤维细胞的培养、传代、冷冻和复苏，观察细胞的形态、生长和贴壁能力。结果显示：组织块培养法培养的细胞，生长较快，呈放射状，体外能正常传代。冷冻和复苏后，细胞能够正常的贴壁、增殖，经胰酶消化后，为核移植实验提供良好的细胞核供体。说明组织块培养方法是简单有效的获得猪耳缘成纤维细胞的方法，获得的细胞可以在体外至少传4代，能够为猪核移植胚胎提供供体细胞。
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| 关 键 词： | 相关分析 相关分析 |
| 收稿时间： | 2012/3/23 0:00:00 |
| 修稿时间： | 2012/4/23 0:00:00 |
The in vitro Culture of Pig Ear Skin Fibroblast |
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| Bo Xu
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Lin Hao
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Liu Yunhai
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Guo Yong
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Ni Hemin.The in vitro Culture of Pig Ear Skin Fibroblast[J].Chinese Agricultural Science Bulletin,2012,28(23):32-35. |
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| Authors: | Bo Xu Lin Hao Liu Yunhai Guo Yong Ni Hemin |
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| Institution: | (College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206) |
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| Abstract: | A method was described for isolation and culture of pig ear skin fibroblast cells defined for porcine somatic nuclear transfer. The experiment was carried out with pig ear skin fibroblasts for primary culture, routine subculture, freezing, and recovery procedure by tissue-pieced method. Those in vitro cultured cells were analyzed by morphology, growth trends and adherent ability. The resuhs showed that pig ear skin fibroblasts was successfully cultured in vitro efficiently, which was with high growth capacity and short passage-period. After freezing and resuscitation, the fibroblasts could also well attach and proliferate, and could be provided as the appropriate donor cells for related nuclear transfer work. The tissue-pieced method was simple and effective access to the pig ear fibroblasts, the cells could be obtained in vitro for at least 4 generations could be used for donor cells for nuclear transfer embryos. |
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| Keywords: | Pig ear skin fibroblast in vitro culture correspondence author |
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