| 一种适用于RT-PCR的拮抗酵母菌总RNA提取方法 |
| |
| 引用本文： | 宋海超,史学群.一种适用于RT-PCR的拮抗酵母菌总RNA提取方法[J].中国农学通报,2012,28(27):199-203. |
| |
| 作者姓名： | 宋海超 史学群 |
| |
| 作者单位： | 海南大学环境与植物保护学院,海口,570228 |
| |
| 基金项目： | 海南省自然科学基金“过表达海藻糖-6-磷酸合成酶基因的拮抗酵母菌转化载体的构建”（807106） |
| |
| 摘 要： |
|
| 关 键 词： | 生态保护 生态保护 |
| 收稿时间： | 2/2/2012 12:00:00 AM |
| 修稿时间： | 2012/6/22 0:00:00 |
An improved method for extracting total RNA used to RT-PCRFrom Antagonistic Yeast Pichia membranefaciens |
| |
| Song Haichao
,
Shi Xuequn.An improved method for extracting total RNA used to RT-PCRFrom Antagonistic Yeast Pichia membranefaciens[J].Chinese Agricultural Science Bulletin,2012,28(27):199-203. |
| |
| Authors: | Song Haichao Shi Xuequn |
| |
| Institution: | (College of Environment and Plant Protection, Hainan University, Haikou 570228) |
| |
| Abstract: | In order to obtain an ideal total RNA extraction method of antagonistic yeast Pichia membranefaciens, three methods, including CTAB method, Trizol method and a modified SDS method were used to extract total RNA from yeast cell and the quality and quantity were compared with ultraviolet spectrophotometer, gel electrophoresis and RT-PCR reaction. The results indicated that, the modified SDS method was better than the other methods since the value of OD260/OD280 and OD260/OD230 were 1.980 and 2.054, the purities were higher, the bands of 28S rRNA, 18SrRNA and 5S rRNA on gel electrophoresis were very clear and did not degraded, the brightness of 28S rRNA bands was twice of the bands of 18S rRNA,and the special band was amplified by RT-PCR. Comparably, CTAB method was not very good as the modified SDS method because the brightness of 28S rRNA bands was similarly as the bands of 18S rRNA. Trizol method was not fit the requirement of RNA extraction, the total RNA degraded and dispersed, DNA pollution was also found on gel electrophoresis. Therefore, the modified SDS method was appropriate for RNA extraction of antagonistic yeast and the quantity was highest. |
| |
| Keywords: | antagonistic yeast total RNA extraction improved SDS method |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
| 点击此处可从《中国农学通报》下载免费的PDF全文 |
|
