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重复超排技术在纯种和牛核心群建立中的应用
引用本文:陈龙,张诺,刘潇,毕江华,余文莉.重复超排技术在纯种和牛核心群建立中的应用[J].中国农学通报,2012,28(26):32-35.
作者姓名:陈龙  张诺  刘潇  毕江华  余文莉
作者单位:1. 河北天和肉牛养殖有限公司,石家庄050051;国家肉牛牦牛产业技术体系石家庄综合试验站,石家庄050051
2. 北京安伯胚胎生物技术中心,北京,100107
基金项目:现代农业产业技术体系建设专项资金“肉牛标准化生产技术研究与示范”(nycytx-38); 国家农业科技成果转化资金项目“应用动物胚胎生物技术快速扩繁纯种日本黑毛和牛核心育种群”(2011GB2A210017); 国家科技支撑计划项目“牛羊高效超排与克隆胚胎生产技术研究与示范”(2011BAD19B02)
摘    要:为了快速扩繁纯种和牛核心群,以最大限度发挥其繁殖潜力。试验以南北方3个典型地区的纯种和牛为试验对象,采用CIDR+FSH+PG方法进行超数排卵,探索合理的重复超排次数及此技术在不同地域环境下的应用状况。高强度重复超排试验表明:初次超排获得的可用胚胎数最理想,随着超排次数的增加,可用胚胎数量有所降低,但是差异不显著(P>0.05);地域间不同超排间隔的超排数据比较,不同地区超排效果差异不显著(7.38±4.27)、(7.88±5.95)、(7.45±5.24)(P>0.05)。在良好的饲养管理前提下,高强度重复超排以1个月为超排间隔,超排3~4次为宜;纯种和牛的适应能力较强,超排间隔在1-2个月,超排3次的常规超排方案可以应用于南北方地区和牛核心群的建立。

关 键 词:硝酸盐  硝酸盐  
收稿时间:2012/5/16 0:00:00
修稿时间:2012/6/14 0:00:00

Establishing of Nucleus Herd of Purebred Wagyu by the Repeated Superovulation
Chen Long , Zhang Nuo , Liu Xiao , Bi Jianghua , Yu Wenli.Establishing of Nucleus Herd of Purebred Wagyu by the Repeated Superovulation[J].Chinese Agricultural Science Bulletin,2012,28(26):32-35.
Authors:Chen Long  Zhang Nuo  Liu Xiao  Bi Jianghua  Yu Wenli
Institution:1 Hebei Tianhe Beef Cattle Farming Ltd,Shijiazhuang 050051 ;2 Shijiazhuang Comprehensive Experiment Station of National Beef Cattle Industrial Technology System,Shijiazhuang 050051 ;3 Beijing Anbo Embryo Biotech Center,Beijing 100107)
Abstract:In order to establish the core group of the purebred Wagyu and maximize their reproductive potential,which from three typical regions in South and North have been superovulated using the method of CIDR + FSH + PG,to explore the reasonable number of repeated superovulation and applied status in the different environment.The results showed that:the experiment of high strength repeated superovulation showed that the most of the transferable embryos have been recovered in the first superovulation,the increased superovulated times have a negative influence on the number of transferable embryos,however,the influence was not significant(P0.05);there were no significant differences among different regions for the transferable embryos(7.38±4.27,7.88±5.95,7.45±5.24,P0.05).In good breeding management,the suitable number of superovulation times were 3 to 4 when the interval was 1 month for high strength repeated super ovulation.The conventional superovulated program,which have 1 to 2 months interval and 3 times could be used to establish the core group of purebred Wagyu in our country.
Keywords:Wagyu  repeated superovulation  superovulation interval  nucleus herd
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