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| 继代培养对球孢白僵菌毒素产生水平的影响 | |
| 引用本文： | 张正坤,孙召朋,张语迟,徐文静,潘洪玉,李启云.继代培养对球孢白僵菌毒素产生水平的影响[J].中国农学通报,2012,28(24):243-249. |
| 作者姓名： | 张正坤 孙召朋 张语迟 徐文静 潘洪玉 李启云 |
| 作者单位： | 1. 吉林省农业科学院,长春,130033 2. 吉林省农业科学院,长春130033;吉林大学,长春130062 3. 长春师范学院,长春,130032 4. 吉林大学,长春,130062 |
| 基金项目： | 基金项目：吉林省科技厅科技引导计划国际科技合作项目“中、俄白僵菌资源遗传多样性研究与利用”（20110751）;吉林省科技厅科技支撑计划重点资助项目“白僵菌高效工程菌的创制、剂型研制及应用”（20080249）. |
| 摘 要： | 继代培养对球孢白僵菌生物学特性、毒力及毒力相关因素等均会产生影响，研究了继代培养对球孢白僵菌毒素产生水平的影响。对野生球孢白僵菌D6-2和L1-1菌株进行室内培养基继代培养，利用高效液相色谱(HPLC)法对其代谢产物中已报道的2种毒素2-氯杀鼠灵酮和间甲基苯甲酸甲酯在不同世代的代谢水平进行了检测。对2株供试球孢白僵菌菌株，收集不同世代在SDAY培养基中培养相同时间后等量的白僵菌培养液，经过有机溶剂提取脂溶性部分，经HPLC定量检测，2个供试菌株各世代培养液中均只有F1代代谢产物中检测出了2-氯杀鼠灵酮，而F2~F5代未检测到，并且供试菌株各世代代谢产物中均未检测到间甲基苯甲酸甲酯；并且发现在继代培养过程中，有未知化合物产生水平未随着世代增加而发生显著变化。不同白僵菌菌株毒素的代谢种类存在差异，并且继代培养会降低某些毒素的代谢水平，也许是继代培养导致供试菌株毒力下降的原因之一；对于其他种类白僵菌毒素在继代培养过程中的代谢水平需进一步研究。 |
| 关 键 词： | 响应面法 响应面法 |
| 收稿时间： | 2011/11/29 0:00:00 |
| 修稿时间： | 2012/2/13 0:00:00 |
Influence on the toxin production level of Beauveria bassiana during subculturing |
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| Zhang Zhengkun , Sun Zhaopeng , Zhang Yuchi , Xu Wenjing , Pan Hongyu , Li Qiyun.Influence on the toxin production level of Beauveria bassiana during subculturing[J].Chinese Agricultural Science Bulletin,2012,28(24):243-249. | |
| Authors: | Zhang Zhengkun Sun Zhaopeng Zhang Yuchi Xu Wenjing Pan Hongyu Li Qiyun |
| Institution: | 1Jilin Academy of Agricultural Sciences, Changchun 130033; 2Jilin University, Changchun 130062; 3Changchun Normal University, Changchun 130032) |
| Abstract: | The biological character, toxicity and toxicity relative factors of Beauveria bassiana were influenced by subculturing, the effect on toxin production level of subculturing was investigated in this research. The B. bassiana strains D6-2 and LI-1 were subcultured on SDAY medium, to detect the influence of subculturing on production level at different generations of toxin methyl 3-methylbenzoate and 2-Coumaranone, by high performance liquid chromatography （HPLC） method. The inoculum at the same culturing time of different B. bassiana generations was collected, and its liposolubility portion was extracted by ethylaleohol and ethyl acetate sequentially, it showed via HPLC detection that 2-Coumaranone was detected in metabolic product of two strains F1 generation, but not in that of F2-F5 generations, and there was no methyl 3-methylbenzoate detected in all samples of the two strains. However, there was other unknown product of metabolism was detected, and established a relative stable expression level in subculturing. There was difference on toxin species of different strains of B. bassiana, and subcuhuring was able to lower the production level of some toxin, it was possible one of the reasons of toxicity decline. It needed further research for the other species of toxin expression level in different subeulturing generations. |
| Keywords: | Beauveria bassiana subeulturing toxin high performance liquid chromatography |
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