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| 朊蛋白在宁夏滩羊不同组织中表达的免疫组化研究 | |
| 引用本文： | 许立华,康晓东,涂健,张珠明,杨建民,周向梅,尹晓敏,杨利峰,赵德明.朊蛋白在宁夏滩羊不同组织中表达的免疫组化研究[J].畜牧兽医学报,2010,41(3). |
| 作者姓名： | 许立华 康晓东 涂健 张珠明 杨建民 周向梅 尹晓敏 杨利峰 赵德明 |
| 作者单位： | 1. 中国农业大学动物医学院国家动物海绵状脑病实验室,北京,100193;宁夏大学农学院,银川,750021 2. 宁夏农林科学院畜产工程技术中心,银川,750004 3. 中国农业大学动物医学院国家动物海绵状脑病实验室,北京,100193 |
| 基金项目： | 科技部星火计划，国家科技支撑计划 |
| 摘 要： | 作者运用免疫组织化学技术,首次对我国一类保护物种宁夏滩羊各组织中朊蛋白(PrP)的分布进行定性、定位研究。结果显示,PrP在滩羊的大脑、脑干、小脑、心脏、肝脏、脾脏、肾脏及淋巴结均有表达,尤其在脾脏、肾脏、脑干、淋巴结中的表达量丰富,而肺脏检测结果为阴性。此研究确定了朊蛋白在滩羊各组织中的表达与分布状况,并优化了朊蛋白检测的技术条件,为羊痒病的诊断与检测提供技术支持。 |
| 关 键 词： | 朊蛋白 组织 表达 免疫组化 滩羊 |
Immunohistochemical Study on PrP Expression in Different Tissues of Tan Sheep in Ningxia |
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| XU Li-hua,KANG Xiao-dong,TU Jian,ZHANG Zhu-ming,YANG Jian-min,ZHOU Xiang-mei,YIN Xiao-min,YANG Li-feng,ZHAO De-ming.Immunohistochemical Study on PrP Expression in Different Tissues of Tan Sheep in Ningxia[J].Acta Veterinaria et Zootechnica Sinica,2010,41(3). | |
| Authors: | XU Li-hua KANG Xiao-dong TU Jian ZHANG Zhu-ming YANG Jian-min ZHOU Xiang-mei YIN Xiao-min YANG Li-feng ZHAO De-ming |
| Institution: | XU Li-hua1,2,KANG Xiao-dong3,TU Jian1,ZHANG Zhu-ming1,YANG Jian-min1,ZHOU Xiang-mei1,YIN Xiao-min1,YANG Li-feng1,ZHAO De-ming 1(1.National Animal Transmissible Spongiform Encephalopathies Laboratory,College of VeterinaryMedicine,China Agricultural University,Beijing 100193,China,2.College of Agriculture,Ningxia University,Yinchuan 750021,3.Technology Center of Animal Products,Ningxia Academy of Agriculture , Forestry Sciences,Yinchuan 750004,China) |
| Abstract: | In the present experiment,a qualitative detection was implemented to determine distribution and location of prion protein(PrP) in different tissues of Ningxia Tan sheep,which is one of the protected species of class I .The result indicated that PrP has a wide range of expression in different tissues,such as cerebrum,brain stem,cerebellum,heart,liver,spleen,kidney,lymph node and so on.Especially,prion protein has a higher expression level in brain stem,spleen,kidney and lymph node than other tissues.We also got a negative result in the samples from lung.This study confirmed the status of distribution of PrP in different tissues from Tan sheep.In addition,the study provided a technical support to diagnose and detect scrapie by optimizing immunohistochemical method. |
| Keywords: | prion protein tissue expression immunohistochemistry Tan sheep |
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