|
|||||
|
|
| 微卫星标记对籼稻品种遗传多样性分析 | |
| 引用本文： | 杨致荣,李润植,魏兴华.微卫星标记对籼稻品种遗传多样性分析[J].山西农业科学,2008,36(8). |
| 作者姓名： | 杨致荣 李润植 魏兴华 |
| 作者单位： | 1. 国家水稻生物学重点实验室,中国水稻研究所,浙江,杭州,310006;山西农业大学生物工程中心,山西,太谷,030501 2. 山西农业大学生物工程中心,山西,太谷,030501 3. 国家水稻生物学重点实验室,中国水稻研究所,浙江,杭州,310006 |
| 摘 要： | 从水稻全基因组12条染色体上的439对SSR引物中选取30对,对国内外的12个籼稻品种进行遗传多样性研究。结果显示,试验选取的30对SSR引物中有29对具有多态性,多态性位点百分率为96.7%。这些多态性引物在12个材料中共扩增出137条多态性条带,平均每对SSR引物可检测到2～8个等位基因,平均4.7/位点。等位基因有效数(Ae)变幅为1～5.539,平均为3.47。多样性指数中,香浓多样性指数(I)为0～0.819,平均0.651;而Nei基因多样性指数变幅为0～1.907,平均1.3。Nei遗传距离及系统聚类和带型分析结果表明,利用SSR标记可将12份材料分为2个类群,国内和国外两个群体。说明SSR标记在区分水稻品种的生态型及遗传多样性研究方面具有重要作用,进一步证实了SSR标记是研究水稻种质资源分类、地理分布、生态类型的有效手段 |
| 关 键 词： | 籼稻 SSR标记 遗传多样性 |
Genetic Diversity Analysis of indica Rice Varieties Based on SSR Markers |
|
| YANG Zhi-rong,LI Run-zhi,WEI Xing-hua.Genetic Diversity Analysis of indica Rice Varieties Based on SSR Markers[J].Journal of Shanxi Agricultural Sciences,2008,36(8). | |
| Authors: | YANG Zhi-rong LI Run-zhi WEI Xing-hua |
| Abstract: | Thirty SSR(simple sequence repeats) primer pairs which comes from 439 SSR that disperse on 12 chromosomes in rice were used to study genetic diversity among 12 indica rice(Oryza sativa L.) varieties.29 of 30 SSR markers generated polymorphic bands,revealing 96.7% polymorphism.A total of 137 polymorphic bands were detected among 12 varieties,The number of alleles(A) per locus range from 2 to 8 with an average of 4.7 alleles.Average effective allele number per locus(Ae) varied from 1 to 5.539 with a mean lever of 3.47.Shannon's index(I)per locus averaged 0.651 and was various from 0 to 0.819.Result from cluster analysis shows that all cultivars could be classified into 2 groups,and this indicated SSR markers play an important role in ecotype classification and study of genetic diversity.Consequently,SSR markers could be used as a useful tool for measuring genetic diversity,assigning rice to geographical distribution,and analysing its ecotype. |
| Keywords: | indica rice SSR Genetic diversity |
| 本文献已被 维普 万方数据 等数据库收录！ | |