| 鸡肠炎沙门氏菌ompA基因的克隆与表达 |
| |
| 引用本文： | 余波,徐景峨,王璇,潭诗文.鸡肠炎沙门氏菌ompA基因的克隆与表达[J].中国畜牧兽医,2012,39(12):20-23. |
| |
| 作者姓名： | 余波 徐景峨 王璇 潭诗文 |
| |
| 作者单位： | 贵州省畜牧兽医研究所,贵州贵阳,550005 |
| |
| 基金项目： | 贵州省科学技术基金项目，贵州省畜禽健康养殖技术创新能力建设项目，中央补助地方科技基础条件专项基金项目 |
| |
| 摘 要： | 根据GenBank中鸡肠炎沙门氏菌ompA基因序列设计1对引物,以鸡肠炎沙门氏菌贵州分离株基因组为模板,扩增鸡肠炎沙门氏菌ompA基因,将其亚克隆到原核表达载体pET-32a(+)中,构建重组表达质粒pET-32a-ompA,转化大肠杆菌BL21(DE3)感受态细胞, 经IPTG诱导, 实现了鸡肠炎沙门氏菌ompA蛋白在大肠杆菌中的表达。SDS-PAGE分析结果表明, 该重组蛋白的分子质量约为55 ku,可溶性分析结果表明表达蛋白大部分以包涵体形式存在。Western blotting分析结果表明,该重组蛋白具备免疫原性。
|
| 关 键 词： | 鸡 肠炎沙门氏菌 ompA基因 原核表达 |
| 收稿时间： | 2012-05-28 |
Cloning and Expression of ompA Gene of Salmonella enteritidis from Chickens |
| |
| YU Bo
,
XU Jing-e
,
WANG Xuan
,
TAN Shi-wen.Cloning and Expression of ompA Gene of Salmonella enteritidis from Chickens[J].China Animal Husbandry & Veterinary Medicine,2012,39(12):20-23. |
| |
| Authors: | YU Bo XU Jing-e WANG Xuan TAN Shi-wen |
| |
| Institution: | Guizhou Institute of Animal Husbandry and Veterinary Medicine, Guiyang 550005,China |
| |
| Abstract: | According to the gene sequences in GenBank of Salmonella enteritidis from chickens, one pair of specific primers was designed for amplifying the specific fragments of ompA gene. The ompA gene was amplified from the isolated Guizhou strain of SE by PCR, and subcloned the sequence into the prokaryotic expression vector pET-32a(+).The recombinant plasmid pET-32a-ompA was transformed into E.coli BL21(DE3) and expressed under the induction of IPTG. The result of SDS-PAGE showed that the expressed protein was about 55 ku, and the recombinant protein was in form of inclusion bodies. Western blotting analysis indicated that this protein had immunological activity. |
| |
| Keywords: | chickens Salmonella enteritidis ompA gene prokaryotic expression |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
