| 绿壳蛋鸡IGF-Ⅰ基因SNPs分析及其与末期产蛋性能的相关性研究 |
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| 引用本文： | 崔梓扬,杨洪雁,张宜强,白秀娟.绿壳蛋鸡IGF-Ⅰ基因SNPs分析及其与末期产蛋性能的相关性研究[J].中国畜牧兽医,2012,39(12):125-128. |
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| 作者姓名： | 崔梓扬 杨洪雁 张宜强 白秀娟 |
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| 作者单位： | 1. 吉林农业大学,吉林长春,130118
2. 东北农业大学动物科学技术学院,黑龙江哈尔滨,150030 |
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| 摘 要： | 本研究以胰岛素样生长因子Ⅰ(insulin-like growth factors-Ⅰ, IGF-Ⅰ)基因作为候选基因,运用PCR-RFLP法检测基因变异,并分析其变异与鸡末期产蛋性能的相关性。以绿壳蛋鸡(n=113)作为试验材料,对IGF-Ⅰ基因5'调控区进行遗传多样性研究。研究结果表明,该调控区扩增产物经PstⅠ酶切后出现AA、AB、BB共3种基因型,经χ2检验绿壳蛋鸡在IGF-Ⅰ位点上符合Hardy-Weinberg平衡状态(P＞0.05)。相关分析表明,IGF-Ⅰ基因5'调控区PstⅠ位点对于产蛋末期(14和15月龄)的产蛋性能没有显著影响(P＞0.05)。
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| 关 键 词： | 绿壳蛋鸡 IGF-Ⅰ基因 产蛋性状 产蛋末期 |
| 收稿时间： | 2012-06-11 |
Single Nucleotide Polymorpisms of IGF-Ⅰ Gene and Association with Late Egg Production in Green Egg Chickens |
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| CUI Zi-yang
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YANG Hong-yan
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ZHANG Yi-qiang
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BAI Xiu-juan.Single Nucleotide Polymorpisms of IGF-Ⅰ Gene and Association with Late Egg Production in Green Egg Chickens[J].China Animal Husbandry & Veterinary Medicine,2012,39(12):125-128. |
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| Authors: | CUI Zi-yang YANG Hong-yan ZHANG Yi-qiang BAI Xiu-juan |
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| Institution: | 1. Jilin Agricultural University, Changchun 130118,China;2. College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China |
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| Abstract: | Considering the association of IGF-Ⅰ with chicken reproductivity, IGF-Ⅰ gene was selected as a candidate to screen the nucleotide variation by PCR-RFLP method and analyze the association of polymorphisms with late egg reproduction. The genetic variation of the 5'region of IGF-Ⅰ gene was detected in 113 Green egg chickens by PCR-RFLP, and three genotypes(AA, BB and AB) were identified through PCR products cutting by PstⅠ restriction enzyme. By Chi square test, black feather Green egg chickens were in Hardy-Weinberg equilibrium at Pst Ⅰ site(P＞0.05). The statistic results showed that laying performance in the telophase of laying (14 and 15 months) was no significant difference between different genotypes(P＞0.05). |
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| Keywords: | Green egg chickens IGF-Ⅰ gene laying performance telophase of laying |
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