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| 基于线粒体DNA控制区(mtDNA D-loop)序列分析贵妃鸡的遗传多样性 | |
| 引用本文： | 吴婵,李辉,李敬瑞,张大权,谢国午,孔嫣,易恒洁.基于线粒体DNA控制区(mtDNA D-loop)序列分析贵妃鸡的遗传多样性[J].中国畜牧兽医,2012,39(12):137-140. |
| 作者姓名： | 吴婵 李辉 李敬瑞 张大权 谢国午 孔嫣 易恒洁 |
| 作者单位： | 1. 贵州省晴隆县草地畜牧中心,贵州晴隆 561400;贵州大学高原山地动物遗传育种与繁殖教育部重点实验室,贵州贵阳550025 2. 贵州大学高原山地动物遗传育种与繁殖教育部重点实验室,贵州贵阳,550025 3. 贵州省晴隆县草地畜牧中心,贵州晴隆,561400 |
| 摘 要： | 采用PCR产物直接测序技术对30只贵妃鸡样品的线粒体DNA控制区(mtDNA D-loop)第Ⅰ高变区序列进行了分析。结果表明,在所分析的D-loop区部分序列中(520 bp),A、G、C、T平均含量分别为26.8%、13.0%、31.1%和29.1%;共发现13个核苷酸多态位点,均为转换位点,未检测到插入/缺失和颠换,核苷酸多样度(Pi)为0.0059,单倍型变异度(Hd)为0.538,中性检验Tajima’s D值为-0.67065。通过群体构建的NJ聚类图分子系统树发现,贵妃鸡起源于红原鸡。 |
| 关 键 词： | 贵妃鸡 线粒体DNA D-loop 遗传多样性 单倍型 |
| 收稿时间： | 2012-06-14 |
Genetic Diversity of Royal Chicken Basesd on Mitochondrial DNA D-loop Region Sequence |
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| WU Chan , LI Hui , LI Jing-rui , ZHANG Da-quan , XIE Guo-wu , KONG Yan , YI Heng-jie.Genetic Diversity of Royal Chicken Basesd on Mitochondrial DNA D-loop Region Sequence[J].China Animal Husbandry & Veterinary Medicine,2012,39(12):137-140. | |
| Authors: | WU Chan LI Hui LI Jing-rui ZHANG Da-quan XIE Guo-wu KONG Yan YI Heng-jie |
| Institution: | 1. Grassland and Animal Husbandry Center of Qinglong County,Qinglong 561400, China;2. Key Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education,Guizhou University,Guiyang 550025,China |
| Abstract: | In this paper, the mitochondrial DNA D-loop sequence(520 bp in length) of 30 individuals from Royal chicken of the first Ⅰ region sequences were analyzed by PCR product direct sequencing technology. The results revealed that the average values of base composition of A,G,C and T in the sequence were 26.8%, 13.0%, 31.1% and 29.1%, respectively. And there were 13 nucleotide polymorphic sites which were transition neither transversion nor insertion/deletion. The haplotype diversity (Hd) and average nucleotide diversity (Pi) of the sites were 0.538 and 0.0059, and the value of Tajima’s D was -0.67065. Using the NJ method showed that the Royal chicken origined in the Red Gallus. |
| Keywords: | Royal chickens mtDNA D-loop genetic diversity haplotype |
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