Abstract: | The study on factors affecting amplification of RAPD for rabbit was carried out by using rabbit genomic DNA as template. The result showed that the concentrations of Taq polymerase, random primer, Mg 2+ and the purity of template DNA played an important role in RAPD analysis.A technical system for RAPD analysis in rabbit was established: in 20μl reaction solution, containing 1×buffer, 2 0mmol/L MgCl 2, 0.2μmol/L primer, 0.1mmol/L dNTP, 90ng template DNA, 1 5UTaq polymerase. |