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绿豆幼苗发育初期叶绿素生物合成的研究
引用本文:张汝民,姚洪军,钟传飞,高荣孚.绿豆幼苗发育初期叶绿素生物合成的研究[J].内蒙古农业大学学报(自然科学版),2005,26(4):30-35.
作者姓名:张汝民  姚洪军  钟传飞  高荣孚
作者单位:北京林业大学林木、花卉遗传育种教育部重点实验室,北京,100083;内蒙古农业大学农学院,呼和浩特,010018;北京林业大学林木、花卉遗传育种教育部重点实验室,北京,100083
基金项目:北京林业大学教育振兴计划人才培养专题(200302014)
摘    要:为了探讨被子植物幼苗发育初期Chl生物合成和调控机制,本文采用不同波长的光照和抗生素处理,分析了光照诱导绿豆黄花幼苗发育初期Chl含量的变化和抗生素对Chl生物合成的影响.结果表明:绿豆黄化幼苗在光照最初4 h,单色光有利于Chl生物合成;光照4 h后,单色光不利于Chl生物合成;同时单色光能够诱导提早Chl b生物合成,BL的作用大于RL1和RL2;脉冲光可以诱导Chl生物合成;抗生素实验显示,在光照2 h内,Chl生物合成过程不受质体和细胞核的调控;光照2 h以后,细胞核对Chl 生物合成有明显的调控作用,光照12 h后叶绿体的发育对Chl生物合成有明显的作用.本实验从亚细胞器水平探讨了绿豆幼苗发育过程Chl生物合成的可能调控机制.

关 键 词:绿豆  光照  抗生素  发育  叶绿素  生物合成
文章编号:1009-3575(2005)04-0030-06
收稿时间:2005-10-14
修稿时间:2005年10月14

STUDY ON CHLOROPHYLL BIOSYNTHESES IN SEEDLING OF PHASEOLUS RADIATUS L. AT EARLY DEVELOPMENT STAGE
ZHANG Ru-min,YAO Hong-jun,ZHONG Chuan-fei,GAO Rong-fu.STUDY ON CHLOROPHYLL BIOSYNTHESES IN SEEDLING OF PHASEOLUS RADIATUS L. AT EARLY DEVELOPMENT STAGE[J].Journal of Inner Mongolia Agricultural University(Natural Science Edition),2005,26(4):30-35.
Authors:ZHANG Ru-min  YAO Hong-jun  ZHONG Chuan-fei  GAO Rong-fu
Institution:1 Key Laboratory of Genetics and Breeding of Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beefing 100083, China College of Agronomy, Inner Mongolia Agricultural University, Hohhot 010018 , China
Abstract:The changes of chlorophyll content and influences of antibiotics on chlorophyll biosynthesis in Phaseolus radiatus seedings were studied under different treatments using fight and antibiotics. Results showed that the monochromatic lights were advantageous to the Chl biosynthesis of etiolution seedling of Phaseolus radiatus in illumination 4 h at first and did not favor after illumination 4 h. The monochromatic light can induce Chl b biosynthesis earlier than the white light, and the effect of blue light is bigger than the red light 1 and red light 2. The pulse light may induce the Chl biosynthesis. The Chl biosynthesis was not regulated by the plastid and nucleus using antibiotic experiments in illumination 2 h, and by the nucleus after illumination 2 h and by the plastid after illumination 12 h. The Chl biosynthesis and regulation mechanism in Phaseolus radiatus seedling were discussed in terms of their subcellular level.
Keywords:Phaseolus radiatus L    illumination  antibiotic  development  chlorophyll  biosynthesis
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