| 哈密瓜细菌性果斑病病原菌鉴定 |
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| 引用本文： | 赵廷昌,孙福在,王兵万,回文广.哈密瓜细菌性果斑病病原菌鉴定[J].植物病理学报,2001,31(4):357-364. |
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| 作者姓名： | 赵廷昌 孙福在 王兵万 回文广 |
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| 作者单位： | 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100094 |
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| 摘 要： | 2000年在内蒙古和新疆的哈密瓜上发现一种新细菌病害-哈密瓜细菌性果斑病,从病叶和病果上分离到33个细菌菌株,接种哈密瓜、西瓜和甜瓜后,发病症状与自然发病症状完全一致,而且从接种病株上又重新分离到了此病原细菌,这33个细菌菌株经柯赫法则证明均为该病的致病菌。各菌株致病力无明显差异。经革兰氏染色反应、菌体形态、培养性状、生理生化反应、细胞化学成分分析(糖、蛋白质、氨基酸、脂肪酸、mol% G+C)、DNA-DNA杂交,确认该病原菌为燕麦食酸菌西瓜亚种(Acidovorax avenae subsp.citrulli Willems et al.1992)=类产碱假单胞菌西瓜亚种(Pseudomonas pseudoalcaligenes subsp.citrulli Schaad et al.1978)。该病菌除侵染哈密瓜外,人工接种尚能侵染多种葫芦科及番茄、茄子等作物。
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| 关 键 词： | 燕麦食酸菌西瓜亚种 哈密瓜果斑病 致病性 鉴定 |
| 文章编号： | 0412-0914(2001)04-0357-08 |
| 收稿时间： | 2000-12-05 |
| 修稿时间： | 2000年12月5日 |
PATHOGEN IDENTIFICATION OF HAMI MELON BACTERIAL FRUIT BLOTCH |
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| ZHAO Ting-chang,SUN Fu-zai,WANG Bing-wan,HUI Wen-guang.PATHOGEN IDENTIFICATION OF HAMI MELON BACTERIAL FRUIT BLOTCH[J].Acta Phytopathologica Sinica,2001,31(4):357-364. |
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| Authors: | ZHAO Ting-chang SUN Fu-zai WANG Bing-wan HUI Wen-guang |
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| Institution: | Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China |
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| Abstract: | A new bacterial disease of Hami melon named as bacterial fruit blotch was first found in Neimenggu and Xinjiang provinces of China in 2000. Thirty three strains were isolated from diseased fruits and leaves of Hami melon. Inoculation on young Hami melon and watermelon seedlings and watermelon and muskmelon fruits with these isolates produced the same symptom as naturally infected plants. All of 33 isolates were identified as Acidovorax avenae subsp.citrulli Willems,Goor,Thieiemans,Gillis,Kersters & Ley(=Pseudomonas pseudoalcaligenes subsp.citrulli Schaad, Sowell, Goth, Colwell &Webb) by pathogenicity, stains reaction, morphological characterization, colony pattern, physiological and biochemical reactions, cytochemical analysis (saccharides, proteins, amino acids,fatty acids and mol% G+C), and DNA DNA hybridization. Inoculations with these strains on leaves of cucumber, Chinese waxgourd, pumpkin, summer squash, watermelon, muskmelon, luffa, balsam pear, vegetable sponge, calabash gourd, green calabash, tomato, and eggplant produced spot symptoms. |
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| Keywords: | Acidovorax avenae subsp citrulli Willems et al 1992 Hami melon bacterial fruit blotch pathogenicity identification |
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