| NaCl胁迫对转甜菜碱醛脱氢酶基因美丽胡枝子生理的影响(英文) |
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| 引用本文： | 杨晓红,陈晓阳,隗晓丹,王颖,周伟,李守菊.NaCl胁迫对转甜菜碱醛脱氢酶基因美丽胡枝子生理的影响(英文)[J].农业科学与技术,2008,9(6):146-150. |
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| 作者姓名： | 杨晓红 陈晓阳 隗晓丹 王颖 周伟 李守菊 |
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| 作者单位： | 杨晓红,YANG Xiao-hong(北京林业大学林木花卉遗传育种教育部重点实验室,北京,100083;北京农学院园林系,北京,102206);陈晓阳,CHEN Xiao-yang(北京林业大学林木花卉遗传育种教育部重点实验室,北京,100083;华南农业大学林学院,广东广州,510642);隗晓丹,王颖,周伟,李守菊,WEI Xiao-dan,WANG Ying,ZHOU Wei,LI Shou-ju(北京农学院园林系,北京,102206) |
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| 基金项目： | 国家高技术研究发展计划(863计划)，北京市科委资助项目，北京农业应用新技术重点实验室开放课题 |
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| 摘 要： | Objective] The aim of this study was to explore the effects of exogenous gene introduction on osmotic adjustment of plants.Method] After pot culture with same time,the transgenic plants expressing betaine aldehyde dehydrogenase gene(BADH)and non-transgenic plants of Lespedeza formosa were treated with different concentrations(0,0.5%,1.0%,2.0%)of NaCl solution for 30 h and some physiological indices including proline,betaine,soluble sugar,malondialdehyde(MDA)contents and superoxide dismutase(SOD)activity were assayed.Result] There was no significant difference on the indices mentioned above between transgenic plants and non-transgenic plants under the salt-free treatment.With the increase of NaCl concentration,the transgenic plants accumulated much higher level of proline,betaine,soluble sugar and lower level of MDA than non-transgenic plants.But the differences of SOD activity increase degrees between transgenic and non-transgenic plants were not significant.Performances of the three transgenic lines were different to some extent.Conclusion] The introduction of exogenous BADH gene enhanced the osmotic adjustment ability of L.formosa,furthermore;different transgenic lines performed differently.
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| 关 键 词： | Lespedeza formosa BADH gene NaCl stress Physiological indices |
Effects of NaCl Stress on Some Physiological Indices of Transgenic Lespedeza formosa Expressing BADH Gene |
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| YANG Xiao-hong,CHEN Xiao-yang,WEI Xiao-dan,WANG Ying,ZHOU Wei,LI Shou-ju.Effects of NaCl Stress on Some Physiological Indices of Transgenic Lespedeza formosa Expressing BADH Gene[J].Agricultural Science ＆ Technology,2008,9(6):146-150. |
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| Authors: | YANG Xiao-hong CHEN Xiao-yang WEI Xiao-dan WANG Ying ZHOU Wei LI Shou-ju |
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| Institution: | 1.Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants,Beijing Forestry University,Beijing 100083;2.Department of Landscape,Beijing University of Agriculture,Beijing 102206;3.College of Forestry,South China Agricultural University,Guangzhou 510642 |
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| Abstract: | Objective] The aim of this study was to explore the effects of exogenous gene introduction on osmotic adjustment of plants. Method] After pot culture with same time, the transgenic plants expressing betaine aldehyde dehydrogenase gene (BADH) and non-transgenic plants of Lespedeza formosa were treated with different concentrations (0, 0.5%, 1.0%, 2.0%) of NaCl solution for 30 h and some physiological indices including proline, betaine, soluble sugar, malondialdehyde (MDA) contents and superoxide dismutase(SOD) activity were assayed. Result] There was no significant difference on the indices mentioned above between transgenic plants and non-transgenic plants under the salt-free treatment. With the increase of NaCl concentration, the transgenic plants accumulated much higher level of proline, betaine, soluble sugar and lower level of MDA than non-transgenic plants. But the differences of SOD activity increase degrees between transgenic and non-transgenic plants were not significant. Performances of the three transgenic lines were different to some extent. Conclusion] The introduction of exogenous BADH gene enhanced the osmotic adjustment ability of L. formosa, furthermore; different transgenic lines performed differently. |
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| Keywords: | Lespedeza formosa BADH gene NaCl stress Physiological indices |
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