| 一株可裂解阴沟肠杆菌的T7样噬菌体的分离鉴定及基因组分析 |
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| 引用本文： | 单文雅,王兆飞,杨登辉,刘云霞,王恒安,严亚贤,孙建和.一株可裂解阴沟肠杆菌的T7样噬菌体的分离鉴定及基因组分析[J].上海交通大学学报(农业科学版),2019(1):74-80, 86. |
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| 作者姓名： | 单文雅 王兆飞 杨登辉 刘云霞 王恒安 严亚贤 孙建和 |
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| 作者单位： | 上海交通大学农业与生物学院上海市兽医生物技术重点实验室 |
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| 基金项目： | 国家自然基金(31772744) |
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| 摘 要： | 利用双层琼脂平板法,从上海地区某奶牛养殖场环境样品中分离、纯化一株针对阴沟肠杆菌的裂解性噬菌体,命名为vB_Ecop_S523(S523),可形成圆形、透明、边缘整齐的噬菌斑。透射电镜(TEM)观察显示,噬菌体头部直径约为58～59 nm,尾部较短,属于Podoviridae科。该噬菌体最佳感染复数为0.1,潜伏期为5 min,裂解量为90 PFU/感染细胞。酶切鉴定S523的核酸类型为双链DNA。测序结果表明,其基因组大小为39,825 bp,G+C含量为48.8%。同源进化分析显示该噬菌体属于T7样噬菌体新成员。上述结果为研究阴沟肠杆菌抑菌制剂提供了新的实验材料。
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| 关 键 词： | 阴沟肠杆菌 T7样噬菌体 生物学特性 基因组分析 |
| 收稿时间： | 2018/7/29 0:00:00 |
Isolation,identification and genomic analysis of a T7-like bacteriophage that can lyse Enterobacter cloacae |
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| SHAN Wen-y,WANG Zhao-fei,YANG Deng-hui,LIU Yun-xi,WANG Heng-an,YAN Ya-xian and SUN Jian-he.Isolation,identification and genomic analysis of a T7-like bacteriophage that can lyse Enterobacter cloacae[J].Journal of Shanghai Jiaotong University (Agricultural Science),2019(1):74-80, 86. |
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| Authors: | SHAN Wen-y WANG Zhao-fei YANG Deng-hui LIU Yun-xi WANG Heng-an YAN Ya-xian and SUN Jian-he |
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| Institution: | Shanghai Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,Shanghai Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,Shanghai Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,Shanghai Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,Shanghai Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,Shanghai Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China and Shanghai Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China |
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| Abstract: | A novel lytic phage against E19 and E20 strains of Enterobacter cloacae,termed vB_Ecop_S523(S523),was isolated from a dairy farm environment sample.The phage has a head of 58-59 nm in diameter and a tail of 10 nm in length,which forms round,transparent and well-defined plaques in the double-layered agar plate.The optimal multiplicity of infection(MOI) of S523 was 0.1,the incubation period was 5 min,and the amount of lysis was about 90 PFU per cell.The S523 was morphologically classified under Podoviridae(T7-like phage)and comprised of double-stranded DNA.The genome of S523 was 39,5 bp(48.8% G+C).Our results indicated S523 could serve as a promising bacteriostatic agent against Enterobacter cloacae. |
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| Keywords: | Enterobacter cloacae T7-like phage biological characteristics genomic analysis |
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