| 表达液胞膜AtNHX1的转基因白三叶提高了耐盐性 |
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| 引用本文： | 赵桂琴,慕平 王锁民 任继周.表达液胞膜AtNHX1的转基因白三叶提高了耐盐性[J].农业生物技术学报,2008,16(5). |
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| 作者姓名： | 赵桂琴 慕平 王锁民 任继周 |
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| 作者单位： | 甘肃农业大学 兰州大学 |
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| 摘 要： | 以白三叶吸胀种子的子叶为转化体，用农杆菌介导法将拟南芥液胞膜Na+/H+ 逆向转运蛋白基因AtNHX1转入白三叶，经过筛选、分化和再生，得到了具有卡那霉素抗性的转基因植株。对这些植株进行PCR、Southern 印迹和 Northern 杂交分析，结果表明，外源目的基因已经整合到白三叶基因组中并且得到了表达。室内耐盐性试验结果表明，表达AtNHX1的转基因植株总叶面积和地上部分干重都显著高于非转基因对照，说明液胞膜上的AtNHX1基因有助于提高白三叶耐盐性。
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| 收稿时间： | 2007-12-29 |
| 修稿时间： | 2008-3-9 |
Transgenic white clover expressing vacuolar AtNHX1 antiporter gene increases salt tolerance |
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| Abstract: | AtNHX1 antiporter gene from Arabidopsis was successfully introduced into white clover plants through their cotyledons, mediated by Agrobacterium tumefaciens. Transgenic plants resistant to Kanamycin were obtained after selection and regeneration. All of the transgenic plants were tested with PCR, Southern blot and Northern analysis, which indicated that AtNHX1 gene has already been integrated into white clover genome and expressed. Indoor salinity test showed that transgenic plants expressing AtNHX1 gene had significantly higher total leaf area and aboveground dry weight compared with non-transgenic controls, illuminating that vacuolar AtNHX1 gene can help to increase salt tolerance in white clover. |
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