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In vitro but not in planta encapsidation of Rice gall dwarf virus core particles by the outer capsid P8 protein of Rice dwarf virus expressed in transgenic rice plants |
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| Authors: | Kyoji Hagiwara Takahiko Higashi Kazunari Takahashi Naho Hara Hideyuki Aoki Naoyuki Miyazaki Qing-Yu Wang Yafeng Zhu Osamu Yatou Hiroshi Tanaka Toshihiro Omura |
| Institution: | (1) National Agricultural Research Center, Tsukuba 305-8666, Japan;(2) National Institute of Agrobiological Sciences, Tsukuba, Japan;(3) Hokuriku Research Center, NARC, Niigata, Japan;(4) Present address: DNAVEC Corporation, Tsukuba, Japan;(5) Present address: The University of Tokyo, Tokyo, Japan;(6) Present address: Hokuriku Research Center, NARC, Niigata, Japan;(7) Present address: Changchun University of Agriculture and Stock Farming, Changchun, China |
| Abstract: | Transencapsidation of the Rice gall dwarf virus (RGDV) inner core by the Rice dwarf virus (RDV) outer capsid P8 protein was examined in vitro and in planta. When RGDV core particles were incubated with an extract from RDV P8-transgenic rice leaf tissue, RDV P8 encapsidated the RGDV core particles to form double-shelled virus-like particles in vitro. In contrast, when RDV P8-transgenic rice plants were inoculated with RGDV, progeny RGDV particles contained RGDV P8 but RDV P8 was not detectable in the virions. No significant differences were found in acquisition by the vector insects and subsequent transmission rates between RGDV infecting nontransgenic rice plants and those infecting RDV P8-transgenic rice plants. These results indicate that mechanisms of and/or requirements for interactions between P8 and the inner core particles of phytoreoviruses differ between in vitro and in planta. |
| Keywords: | Encapsidation Outer capsid protein Rice dwarf virus Rice gall dwarf virus |
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