| 咪唑诱导的人子宫内膜癌细胞HEC-1B空泡化现象研究 |
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| 引用本文： | 张玖航,曾申明.咪唑诱导的人子宫内膜癌细胞HEC-1B空泡化现象研究[J].中国畜牧杂志,2011,47(19). |
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| 作者姓名： | 张玖航 曾申明 |
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| 作者单位： | 中国农业大学动物科技学院,北京,100193 |
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| 摘 要： | 咪唑作为一种弱碱性物质,处理动物子宫内膜细胞后,可诱导其产生空泡化现象,进而影响其生殖生理功能。本研究使用人子宫内膜癌细胞HEC-1B作为模型,使用咪唑处理导致细胞空泡化后探讨空泡化产生的生理机制。通过基因芯片分析、荧光定量PCR检测、激光共聚焦显微镜观察等方法进行研究。结果表明:咪唑处理人子宫内膜癌细胞HEC-1B后,空泡型质子泵(V-ATPase)亚基V0D2基因表达量上调。使用荧光定量PCR验证芯片结果,V-ATPase亚基V0D2 mRNA表达量有所上调。对V-ATPase另外一个亚基V1E1基因进行荧光定量PCR,其mRNA表达量也有所上调。使用V-ATPase抑制剂巴弗洛霉素A1和咪唑共同处理人子宫内膜癌细胞HEC-1B后,通过吖啶橙染色,激光共聚焦显微镜观察,咪唑诱导的空泡被显著抑制。上述结果证明了V-ATPase在咪唑诱导的细胞空泡化中的重要作用。
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| 关 键 词： | 空泡型质子泵 细胞空泡化 咪唑 人子宫内膜癌细胞HEC-1B |
Study on the Vacuolation of Imidazole-Treated Human Endometrial Carcinoma Cell Line HEC-1B |
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| ZHANG Jiu-hang
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ZENG Shen-ming.Study on the Vacuolation of Imidazole-Treated Human Endometrial Carcinoma Cell Line HEC-1B[J].Chinese Journal of Animal Science,2011,47(19). |
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| Authors: | ZHANG Jiu-hang ZENG Shen-ming |
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| Institution: | ZHANG Jiu-hang,ZENG Shen-ming(College of Animal Science and Technology,China Agriculturd University,Beijing 100193,China) |
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| Abstract: | As a kind of weak bases,imidazole cause vacuolation of animal endometrial cells,and affect the physiological and reproductive functions.Our study discuss the mechanism of vacuolation after treatment with imidazole through experimental model of human endometrial carcinoma cell line HEC-1B.We study through Genechip research,fluorescence quantitative PCR and observation of laser confocal microscopy.Results:Genechip research results show that gene of subunit V0D2 of human vacuolar type H+-ATPase(V-ATPase) had a... |
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| Keywords: | vacuolar type H -ATPase(V-ATPase) vacuolation imidazole human endometrial carcinoma cell line HEC-1B |
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