| N-乙酰半胱氨酸对脂多糖刺激仔猪小肠黏膜免疫应激的影响 |
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| 引用本文： | 杨震国,张伟,侯永清,丁斌鹰,王蕾,刘玉兰,朱惠玲.N-乙酰半胱氨酸对脂多糖刺激仔猪小肠黏膜免疫应激的影响[J].中国畜牧杂志,2011,47(23). |
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| 作者姓名： | 杨震国 张伟 侯永清 丁斌鹰 王蕾 刘玉兰 朱惠玲 |
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| 作者单位： | 武汉工业学院动物营养与饲料科学湖北省重点实验室,湖北武汉,430023 |
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| 基金项目： | 武汉市学术带头人计划项目(200951830554); 湖北省自然科学基金计划创新群体项目(2007ABC009); 湖北省高等学校优秀中青年团队计划项目(T200810) |
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| 摘 要： | 研究N-乙酰半胱氨酸(NAC)对脂多糖(LPS)刺激仔猪免疫应激的影响。选取18头健康仔猪,随机分成3个处理(对照组、LPS组和NAC组),每个处理6个重复。对照组和LPS组饲喂基础日粮,NAC组饲喂基础日粮+500 mg/kgNAC。试验期为20 d。LPS组和NAC组于试验第10、13、20天腹膜注射100μg/kg BW的LPS,对照组注射相应剂量的灭菌生理盐水。第10、20天注射LPS后3 h采血,第21天屠宰,取小肠黏膜。结果表明:NAC缓解了LPS刺激导致的血浆和小肠黏膜中TNF-α、IL-6、PGE2含量的升高(P<0.05),缓解了小肠黏膜HSP70相对表达量的升高(P<0.05)。由此可见,日粮中添加500 mg/kg NAC可有效抑制LPS刺激导致的血浆及小肠黏膜中炎性因子的升高,缓解免疫应激。
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| 关 键 词： | N-乙酰半胱氨酸 脂多糖 免疫应激 炎性介质 仔猪 |
Effects of N-acetylcysteine on Intestinal Mucosal Immunological Stress in Piglets Chronically Challenged with Lipopolysaccharide |
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| YANG Zhen-guo
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ZHANG Wei
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HOU Yong-qing
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DING Bin-ying
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WANG Lei
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LIU Yu-lan
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ZHU Hui-ling.Effects of N-acetylcysteine on Intestinal Mucosal Immunological Stress in Piglets Chronically Challenged with Lipopolysaccharide[J].Chinese Journal of Animal Science,2011,47(23). |
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| Authors: | YANG Zhen-guo ZHANG Wei HOU Yong-qing DING Bin-ying WANG Lei LIU Yu-lan ZHU Hui-ling |
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| Institution: | YANG Zhen-guo,ZHANG Wei,HOU Yong-qing*,DING Bin-ying,WANG Lei,LIU Yu-lan,ZHU Hui-ling(Hubei Key Laboratory of Animal Nutrition and Feed Science,Wuhan Polytechnic University,Hubei Wuhan 430023,China) |
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| Abstract: | The aim of the current study was to evaluate effects of dietary supplementation with N-acetylcysteine(NAC)on intestinal mucosal immunological stress with reference to its molecular mechanism in piglets after lipopolysaccharide(LPS) challenge.Eighteen healthy piglets with the same genetic origin(Duroc ×Landrace × Large white) and similar body weight(11.58 ± 0.26 kg BW) were randomly allocated to 3 treatments(control group,LPS group and NAC group).Each treatment included 6 replicates,one piglet per replicate.The piglets in the control group and LPS group were fed with the basal diet,while the NAC group was fed with the basal diet + 500 mg/kg NAC.The feeding period was 20 days.On 10,13 and 20 d,the piglets in the LPS and NAC groups were injected intraperitoneally with 100 μg/kg BW LPS,and the piglets in the control group were injected intraperitoneally with the same dose of physiological saline.On 10 and 20 d,blood samples were collected from precava vein after 3 h post-injection of LPS.On 21 d,all the piglets were sacrificed to collect jejunal and ileal mucosa for analysis.The results showed that dietary supplementation of NAC significantly attenuated the increase of TNF-α,IL-6 and PGE2 in plasma and,jejunal,ileal mucosa,the increase of TNF-α and COR in duodenal mucosa,induced by LPS challenge(P<0.05),attenuated the increase of the expression of HSP70 induced by LPS challenge(P<0.05),trended to attenuate the increase of IL-6 in in duodenal mucosa(P<0.1).Under the present experimental conditions,dietary supplementation with 500 mg/kg NAC inhibited increases of inflammatory mediums in plasma and intestinal mucosa,alleviated immunological stress. |
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| Keywords: | N-acetylcysteine lipopolysaccharide immunological stress inflammatory mediums piglets |
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