| 奶牛乳腺上皮细胞的原代培养(英文) |
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| 引用本文： | 吴娟,王凤龙,王申元.奶牛乳腺上皮细胞的原代培养(英文)[J].Agricultural Science&Technology,2009(1). |
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| 作者姓名： | 吴娟 王凤龙 王申元 |
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| 作者单位： | 内蒙古农业大学动物科学与医学学院病理研究室; |
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| 基金项目： | Supported by Natural Science Foundation of Inner Mongolia Autono-mous Region (200711020407);;China Agricultural University and Inner Mongolia Agricultural University Cooperation Projects~~ |
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| 摘 要： | Objective] To investigate the feasibility of the primary culture of bovine mammary epithelial cells in biochemical incubator. Method] In vitro, bovine mammary epithelial cells were isolated and cultured by the tissue explant method in order to investigate the optimal culture conditions. The morphology observation and identification of the cultured cells were performed by inverted microscope observation, Giemsa staining and cytokeratin immunohistochemistry. Result] Observed with inverted microscope, most of the bovine mammary epithelial cells were polygonal and displayed typical slabstone-like appearance. As it can be seen from cell staining results, the cell body was big and the nucleus was stained dark blue and was round or oval in shape, with clearly visible nucleoli, generally 2-4 nucleoli. The tissue-specific expression of cytokeratin 14 and cytokeratin 18 genes in mammary epithelial cells was identified by cytokeratin immunohistochemistry. Conclusion] Primary bovine mammary epithelial cells were successfully cultured in biochemical incubator.
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| 关 键 词： | 培养的 细胞角蛋白 成纤维细胞 乳腺上皮细胞 组织块 奶牛 乳腺组织 原代培养 免疫组化 |
Primary Culture of Bovine Mammary Epithelial Cells |
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| WU Juan,WANG Feng-long,WANG Shen-yu,epartment of Pathology,College of Animal Science , Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot.Primary Culture of Bovine Mammary Epithelial Cells[J].农业科学与技术(英文版),2009(1). |
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| Authors: | WU Juan WANG Feng-long WANG Shen-yu epartment of Pathology College of Animal Science Veterinary Medicine Inner Mongolia Agricultural University Hohhot |
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| Institution: | WU Juan,WANG Feng-long,WANG Shen-yu,epartment of Pathology,College of Animal Science , Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018 |
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| Abstract: | Objective] To investigate the feasibility of the primary culture of bovine mammary epithelial cells in biochemical incubator. Method] In vitro, bovine mammary epithelial cells were isolated and cultured by the tissue explant method in order to investigate the optimal culture conditions. The morphology observation and identification of the cultured cells were performed by inverted microscope observation, Giemsa staining and cytokeratin immunohistochemistry. Result] Observed with inverted microscope, most ... |
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| Keywords: | Bovine mammary epithelial cells Primary culture Cells growing on cover slip Immunohistochemistry |
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