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| 一种筛选稗草生防潜力菌的简易生测方法 | |
| 引用本文： | 黄世文,余柳青,段桂芳,李迪,赵航,乔凤云,喻锦秀,罗宽.一种筛选稗草生防潜力菌的简易生测方法[J].中国生物防治,2004,20(1):53-56. |
| 作者姓名： | 黄世文 余柳青 段桂芳 李迪 赵航 乔凤云 喻锦秀 罗宽 |
| 作者单位： | 1. 中国水稻研究所,杭州,310006;湖南农业大学,长沙,410128 2. 中国水稻研究所,杭州,310006 3. 湖南农业大学,长沙,410128 |
| 基金项目： | 国家自然科学基金 (30 0 70 5 0 5 )，浙江省重点资助项目 (0 1110 2 4 6 9) |
| 摘 要： | 将催芽至露白的稗草和水稻种子直接接种在于PDA培养基上培养形成的 4种稗草病原菌 (交链孢菌、弯孢菌、禾长蠕孢菌稗草专化型、尖角突脐孢菌 )菌落上 ,观察稻、稗苗感病情况 ,调查根、芽生长抑制率。结果表明 ,4种菌对稻、稗的致病性和生长抑制率与常规生测法结果基本吻合。说明在培养基中初步测定、筛选稗草生防潜力菌是可行的。该方法与常规生测法比较 ,可节省一半时间左右 ,同时可减少工作量。 |
| 关 键 词： | 稗草病原菌 简易生测法 致病性 生长抑制率 |
| 文章编号： | 1005-9261(2004)01-0053-04 |
| 修稿时间： | 2003年1月20日 |
A Simple Bioassay Method for Screening Pathogenic Fungi to Weeds |
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| HUANG Shi-wen,YU Liu-qing,DUAN Gui-fang,LI Di,ZHAO Hang,QIAO Feng-yun,YU Jin-xiu,LUO Kuan.A Simple Bioassay Method for Screening Pathogenic Fungi to Weeds[J].Chinese Journal of Biological Control,2004,20(1):53-56. | |
| Authors: | HUANG Shi-wen YU Liu-qing DUAN Gui-fang LI Di ZHAO Hang QIAO Feng-yun YU Jin-xiu LUO Kuan |
| Abstract: | Alternaria alternata, Curvularia lunata, Helminthosporium gramineum Rabenh f. sp. echinochloae and Exserohilum monoceras are pathogenic fungi of barnyardgrass (Echinochloa crus-galli). Germinated seeds (coleoptile and radicle just emerged) of barnyardgrass and rice were directly inoculated on colonies of the pathogenic fungi cultured on PDA plates. Infection and growth inhibition of barnyardgrass and rice seedlings were investigated after culturing for several days. Results showed that the pathogenicity and growth inhibition of barnyardgrass by the four pathogens were almost the same with the results of normal test. Comparing to the normal test, the bioassay can save half time and reduce workload for testing and screening. |
| Keywords: | barnyardgrass pathogens simple bioassay pathogenicity growth inhibition rate |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |