| 不同提取方法对RA外膜蛋白SDS-PAGE的影响 |
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| 引用本文： | 杨加琼.不同提取方法对RA外膜蛋白SDS-PAGE的影响[J].中国农学通报,2012,28(14):62-65. |
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| 作者姓名： | 杨加琼 |
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| 作者单位： | 眉山职业技术学院,四川眉山,620036 |
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| 摘 要： | 分别用Sds法、Sarkosyl法、PMSF法提取鸭疫里氏杆菌的外膜蛋白，经SDS－PAGE电泳，显示，三种方法都能提取到鸭疫里氏杆菌的外膜蛋白，但电泳条带却有一定差异；相比之下，Sds法最适于鸭疫里氏杆菌外膜蛋白的提取；几种方法提取的其他分子量外膜蛋白质是否属于鸭疫里氏杆菌的外膜蛋白抗原需要进一步研究。
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| 关 键 词： | 新农村建设 新农村建设 农民 文化 文化权益 |
| 收稿时间： | 3/4/2011 12:00:00 AM |
| 修稿时间： | 2011/6/17 0:00:00 |
Influence of different extracted methods on the outer membrane protein of RA SDS-PAGE electrophoresis |
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| Yang Jiaqiong.Influence of different extracted methods on the outer membrane protein of RA SDS-PAGE electrophoresis[J].Chinese Agricultural Science Bulletin,2012,28(14):62-65. |
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| Authors: | Yang Jiaqiong |
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| Institution: | Yang Jiaqiong(Meishan Vocational and Technical College,Meishan Sichuan 620036) |
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| Abstract: | The outer membrane of the Riemerella Anatipestifer(abbreviate as RA) are extracted through Sds method,Sarkosyl method,PMSF method,and were electrophoresized respectively.Test result showed that three methods could extract the outer membrane of RA,but display differently electrophoresis bands by Coomassie brilliant stained;by comparison,Sds method is most suitable for extracting the outer membrane of RA;whether or other proteins whose molecular weight are different by the three methods belong the outer membrane protein antigeon,which need to be research farther more. |
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| Keywords: | RA outer membrane protein SDS-PAGE electrophoresis |
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