Similarities and differences between Amaranthus species and cultivars and estimation of outcrossing rate on the basis of electrophoretic separations of urea-soluble seed proteins |
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| Authors: | Jerzy Drzewiecki |
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| Institution: | (1) Department of Agronomy, Korea University, Anam-dong, Seongbuk-Gu, Seoul, 136-701, Korea;(2) Department of Crop & Soil Sciences, University of Georgia, Griffin, GA, 30223, U.S.A. |
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| Abstract: | Wheat-rye translocation line (2BS/2RL) has been developed for resistance to biotype L of Hessian fly and agronomically useful
traits. AFLP analysis using 64 primer pairs was conducted in order to identify 2RL-specific polymorphisms between “Coker 797”
(non-2RL), near-isogenic line (NIL) carrying 2RL, and “Hamlet”. Nine primer combinations identified twelve reproducible polymorphic
fragments in the NIL carrying 2RL. These twelve fragments were cloned and sequenced with an aim towards converting AFLP markers
into sequence tagged sites (STS). A comparison of the 12 sequences with non-redundant accessions in the NCBI database using
the BLAST search option indicated that one fragment of approximately 200 bp in length (amplified using primer combination
E+AAC / M+CTA) was highly homologous with the rye-specific repetitive sequence R173-1 and Wis-2-lA, a retrotransposon-like
element in wheat. Two STS primers (SJ07 and SJ09) out of twelve STS primer sets enabled the detection of polymorphisms between
Coker 797 and NIL carrying 2RL. In order to verify whether the polymorphism detected by primers SJ07 and SJ09 was in fact
the result of the presence of 2RL, additional plant material was examined. Amplified products of about 260 bp fragment with
the SJ07 primer set were generated in rye cvs.“Chilbohomil” and “Jochunhomil”, triticale experimental line Suwon 15, and wheat
experimental line K-14 (1AL/1RS & 2BS/2RL), as well as NIL carrying 2RL and Hamlet, but not in Coker 797 (non-2RL), “Keumgangmil”
(non-translocation wheat), KS92WGRC17 (PI592729 /;/ 6BS/6BL-6RL), KS92WGRC19 (P1592731 /;/ 4BS/4BL-6RL), “TAM200” (1AL/1RS),
and “Siouxland” (1BL/1RS). Our data suggest that primer set SJ07 amplifies a “2RL-specific” fragment of diagnostic value.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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| Keywords: | 2BS/2RL AFLP H21 molecular markers STS wheat-rye translocation |
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