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| 犬脾细胞白介素-18(IL-18)基因的克隆与序列分析 | |
| 引用本文： | 曹殿军,于立辉,闫丽辉,刘培欣,孙建宏,杨玉英,赵玉军,杨国勇.犬脾细胞白介素-18(IL-18)基因的克隆与序列分析[J].中国预防兽医学报,2003,25(4):262-264. |
| 作者姓名： | 曹殿军 于立辉 闫丽辉 刘培欣 孙建宏 杨玉英 赵玉军 杨国勇 |
| 作者单位： | 1. 中国农业科学院,哈尔滨兽医研究所,兽医生物技术,黑龙江,哈尔滨,150001 2. 中国农业科学院,哈尔滨兽医研究所,兽医生物技术,黑龙江,哈尔滨,150001;沈阳农业大学,辽宁,沈阳,110160 3. 沈阳农业大学,辽宁,沈阳,110160 |
| 摘 要： | 为了研究白细胞介素-18(IL-18)的活性和功能，将犬脾细胞经ConA诱导，用TRIzol裂解，提取总RNA，通过反转录聚合酶链反应(RT-PCR)扩增出犬IL-18的cDNA，然后连接到pMD18-T载体上，转化DH5a感受态细胞，获得阳性重组质粒。核苷酸序列结果表明，我们得到了长686bp的基因，含有一个582bp的开放阅读框架，编码193个氨基酸。与已知犬序列同源性可高达99．6％，与已知猪序列的同源性为89．8％，所克隆基因为今后进一步研究IL-18基因的功能奠定了基础。 |
| 关 键 词： | 犬 脾细胞IL－18基因 克隆 测序 |
| 文章编号： | 1008-0589(2003)04-0262-03 |
| 修稿时间： | 2002年12月20 |
Cloning and sequencing interleukin-18 from dog spleen |
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| CAO Dian_jun ,YU Li_hui ,YAN Li_hui ,LIU Pei_xin ,SUN Jian_hong ,YANG Yu_ying ,ZHAO Yu_jun ,YANG Guo_yong.Cloning and sequencing interleukin-18 from dog spleen[J].Chinese Journal of Preventive Veterinary Medicine,2003,25(4):262-264. | |
| Authors: | CAO Dian_jun YU Li_hui YAN Li_hui LIU Pei_xin SUN Jian_hong YANG Yu_ying ZHAO Yu_jun YANG Guo_yong |
| Institution: | CAO Dian_jun 1*,YU Li_hui 1,2,YAN Li_hui 1,LIU Pei_xin 1,SUN Jian_hong 1,YANG Yu_ying 2,ZHAO Yu_jun 2,YANG Guo_yong 2 |
| Abstract: | To provide a basis for further investigation of the function of dog IL_18,a pair of primers specific to dog Interleukin_18 (IL_18)were designed and synthesized according to the known sequence from Genbank, and were used to amplify dog IL_18 cDNA by RT_PCR from total RNA of dog spleen cells induced with ConA. RT_PCR product was cloned into pMD18_T vector. The nucleotide acid sequence had been determined after being identified by restriction endonucleases digestion. The result showed that cloned IL_18 cDNA was 686 bp in length with a 582 bp open read frame encode a polypeptide of 193 amino acid.Compared with published dog IL_18, nucleotide acid and amino acid homology are 99.6 % and 99.8 % respectively. Cloned dog IL_18 showed 89.8 % homology with pig IL_18. |
| Keywords: | dog spleen cells interleukin_18 cloning nucleotide sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |