| 基于线粒体D-loop区探讨我国养殖大口黑鲈的分类地位和遗传变异 |
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| 引用本文： | 李胜杰,白俊杰,叶星,简清,宋红梅.基于线粒体D-loop区探讨我国养殖大口黑鲈的分类地位和遗传变异[J].海洋渔业,2008,30(4). |
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| 作者姓名： | 李胜杰 白俊杰 叶星 简清 宋红梅 |
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| 作者单位： | 中国水产科学研究院珠江水产研究所,中国水产科学研究院热带亚热带鱼类选育与养殖重点开放实验室,广州,510380 |
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| 基金项目： | 广东省重大科技兴渔项目
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国家科技支撑计划
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中国水产科学研究院水产种质资源与养殖技术重点开放实验室开放课题
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| 摘 要： | 对大口黑鲈国内养殖种群(G)和北方亚种(N)、佛罗里达亚种(F)两个野生种群共23个个体的线粒体DNA D-loop区序列进行分析,探讨国内养殖大口黑鲈(Micropterus salmoides)的分类地位和遗传变异。D-loop区序列分析结果表明,共检测到73个变异位点,总变异率为9.0%。三群体间没有共享单倍型,群体G的5个个体含2种单倍型,群体N的11个个体含9种单倍型,群体F中每个个体均为一种单倍型。对三个群体间的遗传距离分析表明,养殖群体与北方亚种野生群体的遗传距离为0.009,与佛罗里达亚种野生群体的遗传距离为0.053,表明国内养殖的大口黑鲈在分类上属于北方亚种,分子系统进化树的进一步分析结果与其一致。群体N、F和G的核苷酸多样性指数(π)依次为0.008 2,0.013和0.000 5,单倍型多样性指数(h)分别为0.946,1.000和0.400,显示出养殖大口黑鲈群体的遗传多样性相比国外野生群体有明显下降,有必要开展大口黑鲈的遗传改良研究,提高其种质质量。
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| 关 键 词： | 大口黑鲈 D-loop 分类地位 遗传多样性 |
Approach on the taxonomic status and genetic variation of largemouth bass(Micropterus salmoides) cultured in China based on mitochondrial D-loop gene |
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| LI Sheng-jie,BAI Jun-jie,YE Xing,JIAN Qing,Song Hong-mei.Approach on the taxonomic status and genetic variation of largemouth bass(Micropterus salmoides) cultured in China based on mitochondrial D-loop gene[J].Marine Fisheries,2008,30(4). |
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| Authors: | LI Sheng-jie BAI Jun-jie YE Xing JIAN Qing Song Hong-mei |
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| Institution: | LI Sheng-jie,BAI Jun-jie,YE Xing,JIAN Qing,Song Hong-mei(Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Tropical & Subtropical Fish Breeding& Cultivation,CAFS,Guangzhou 510380,China) |
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| Abstract: | Using nucleotide sequences from control region(D-loop) of mitochondrial,the taxonomic status and genetic variation of largemouth bass cultured population in China have been approached.The results from sequence analysis of 23 samples including largemouth bass cultured population(G) in China and two wild populations for northern subspecies(N) and Florida subspecies(F) both from America revealed that there were 73 variable sites(9%) among 810 bp.Among three populations,there was no shared haplotype to be detec... |
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| Keywords: | D-loop |
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