| 利用响应面法优化全蚀病生防菌YB-81的发酵条件 |
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| 引用本文： | 全鑫,杨艳艳,杨丽荣,武超,薛保国.利用响应面法优化全蚀病生防菌YB-81的发酵条件[J].天津农业科学,2014(9):57-61. |
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| 作者姓名： | 全鑫 杨艳艳 杨丽荣 武超 薛保国 |
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| 作者单位： | 河南省农业科学院植物保护研究所农业部华北南部作物有害生物综合治理重点实验室河南省农作物病虫害防治重点实验室,河南郑州450002 |
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| 基金项目： | 农业部十二五科技支撑课题（2012BAD19B04）;河南省农科院自主创新基金项目 |
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| 摘 要： | 为了优化全蚀病生防菌株YB-81的发酵培养基及培养条件,提高其对全蚀病的抑制效果,在单因素试验的基础上,利用响应面法(Response surface methodology)对小麦全蚀病生防菌株YB-81的发酵条件进行优化。生防菌株YB-81的最优发酵条件为:时间36h,温度36℃,初始pH值8.0,转速200 r·min-1,牛肉膏+葡萄糖(1∶1)0.53%,蛋白胨0.99%,氯化钠0.20%。在此培养条件下试验,其菌落总数为18.8亿个·mL-1,较基础培养基提高了72%,对全蚀病菌抑制率达到87.2%,较优化前提高了32.5%。生防菌YB-81的最优发酵培养条件的确定,可为该菌大规模生产应用提供理论基础。
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| 关 键 词： | 小麦全蚀病 生防菌 响应面法 发酵 |
Optimization of Fermentaion Conditions of the Biocontrol Strain YB-81 Against Gaeumannomyces gramims Using Response Surface Methodology |
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| QUAN Xin,YANG Yan-yan,YANG Li-rong,WU Chao,XUE Bao-guo.Optimization of Fermentaion Conditions of the Biocontrol Strain YB-81 Against Gaeumannomyces gramims Using Response Surface Methodology[J].Tianjin Agricultural Sciences,2014(9):57-61. |
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| Authors: | QUAN Xin YANG Yan-yan YANG Li-rong WU Chao XUE Bao-guo |
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| Institution: | (IPM Key Laboratory in Northern-South of the Ministry of Agriculture, I-Ienan Key Laboratory for Control of Crop Diseases and Insect Pests, Henan Academy of Agricultural Sciences, Zhengzhou,Henan 450002, China) |
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| Abstract: | In order to raising the antagonistic effect, the growth conditions and culture medium of biocontrol strain YB-81 was opti- mized based on single factor test. The optimal culture conditions of the biocontrol strain YB-81 were determined for 0.53% beef ex- tract＋glucose （1：1）, 0.99% peptone, 0.20% odium chloride, temperature at 36 %, pH 8.0, speeding at 200 r. min-1, the incubation time of 36 h. The total numbers of colony was 1.88×10^9·mL-1, improving by 72%, and the inhibition rate to Gaeumannomyces gramims was 87.2%, improving by 32.5% with the optimal growth conditions. The optimal growth condition of the biocontrol strain YB-81 was identified, providing a theoretical basis for enlargement of production. |
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| Keywords: | wheat take-all biocontrol strain response surface methodology fermentation |
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