Translating biomolecular recognition into nanomechanics |
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| Authors: | Fritz J Baller M K Lang H P Rothuizen H Vettiger P Meyer E Güntherodt H Gerber C Gimzewski J K |
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| Institution: | IBM Research, Zurich Research Laboratory, S?umerstrasse 4, CH-8803 Rüschlikon, Switzerland. |
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| Abstract: | We report the specific transduction, via surface stress changes, of DNA hybridization and receptor-ligand binding into a direct nanomechanical response of microfabricated cantilevers. Cantilevers in an array were functionalized with a selection of biomolecules. The differential deflection of the cantilevers was found to provide a true molecular recognition signal despite large nonspecific responses of individual cantilevers. Hybridization of complementary oligonucleotides shows that a single base mismatch between two 12-mer oligonucleotides is clearly detectable. Similar experiments on protein A-immunoglobulin interactions demonstrate the wide-ranging applicability of nanomechanical transduction to detect biomolecular recognition. |
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