| 部分烟草种质亲缘关系的AFLP分析 |
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| 引用本文： | 杜传印,刘洪祥,田纪春.部分烟草种质亲缘关系的AFLP分析[J].作物学报,2006,32(10):1592-1596. |
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| 作者姓名： | 杜传印 刘洪祥 田纪春 |
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| 作者单位： | 1山东省作物生物学重点实验室/山东农业大学农学院，山东泰安271018；2中国烟草总公司青州烟草研究所，山东青岛266101 |
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| 基金项目： | 科技部社会公益研究项目 |
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| 摘 要： | 利用AFLP技术,对48份不同类型和地域来源的烟草种质的亲缘关系进行了分析。选用4对AFLP引物共检测到323条扩增带,其中174条具有多态性,平均多态检出率为55.72%。对AFLP扩增结果采用UPGMA法进行聚类分析,可以将48份烟草种质分为2大类群,即黄花烟草群和普通烟草群,后者又可进一步分为4组;48份材料的欧氏距离为1.41~10.78。初步研究表明,AFLP标记技术能较好地从分子水平揭示烟草种质资源的遗传背景和亲缘关系。
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| 关 键 词： | 烟草 种质资源 亲缘关系 AFLP 聚类分析 |
| 收稿时间： | 2005-11-04 |
| 修稿时间： | 2006-03-12 |
Phylogenetic Analysis of Partial Tobacco Germplasm Resources with AFLP Markers |
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| DU Chuan-Yin,LIU Hong-Xiang,TIAN Ji-Chun.Phylogenetic Analysis of Partial Tobacco Germplasm Resources with AFLP Markers[J].Acta Agronomica Sinica,2006,32(10):1592-1596. |
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| Authors: | DU Chuan-Yin LIU Hong-Xiang TIAN Ji-Chun |
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| Institution: | 1.Key Laboratory of Crop Biology of Shandong Province/Agronomy College, Shandong Agricultural University, Tai’an 271018, Shandong;2.Qingzhou Tobacco Research Institute of CNTC，Qingdao 266101, Shandong, China |
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| Abstract: | Phylogenesis of 48 tobacco germplasms from different countries or regions was analyzed with amplified fragment length polymorphism(AFLP).Four pairs of AFLP informative primers generated 323 scorable bands in total,in which 174 bands were polymorphic and the average percentage of polymorphic bands was 55.72.Cluster analysis based on Euclidean distance by unweighted pair-group method using arithmetic average(UPGMA) clearly separated the 48 germplasms into two groups,namely N.tabacum L.group and N.rustica L.group,and further the N.tabacum L.group was subdivided into four sections.The Euclidean distances among the 48 germplasms ranged from 1.41 to 10.78.These results suggest that AFLP technique can be used to reveal the genetic background and phylogenetic relationship of tobacco germplasm. |
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| Keywords: | AFLP |
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