| 长丰鲢生长性状相关微卫星标记的初步筛选 |
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| 引用本文： | 吴新燕,梁宏伟,罗相忠,王丹,沙航,邹桂伟.长丰鲢生长性状相关微卫星标记的初步筛选[J].水产学报,2022,46(1):20-30. |
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| 作者姓名： | 吴新燕 梁宏伟 罗相忠 王丹 沙航 邹桂伟 |
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| 作者单位： | 中国水产科学研究院长江水产研究所,湖北武汉430223;南京农业大学无锡渔业学院,江苏无锡214081;中国水产科学研究院长江水产研究所,湖北武汉430223 |
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| 基金项目： | 现代农业产业技术体系建设专项资金(CARS-45-01); 国家淡水水产种质资源库项目 |
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| 摘 要： | 为了筛选出与长丰鲢生长性状相关的分子标记,以指导其种质鉴定和选择育种工作,实验利用15个多态微卫星标记,对6、17和36月龄长丰鲢进行了遗传多样性和生长性状关联分析.结果 显示,各月龄长丰鲢的观测杂合度(Ho)和期望杂合度(Hc)均高于0.5,PIC均值分别为0.555、0.445和0.490,表明各月龄长丰鲢群体均具...
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| 关 键 词： | 长丰鲢 微卫星标记 生长性状 相关性分析 |
| 收稿时间： | 2020/12/13 0:00:00 |
| 修稿时间： | 2021/2/4 0:00:00 |
Preliminary screening of microsatellite DNA markers associated with growth traits in Changfeng silver carp (Hypophthalmichthys molitrix) |
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| WU Xinyan,LIANG Hongwei,LUO Xiangzhong,WANG Dan,SHA Hang,ZOU Guiwei.Preliminary screening of microsatellite DNA markers associated with growth traits in Changfeng silver carp (Hypophthalmichthys molitrix)[J].Journal of Fisheries of China,2022,46(1):20-30. |
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| Authors: | WU Xinyan LIANG Hongwei LUO Xiangzhong WANG Dan SHA Hang ZOU Guiwei |
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| Institution: | Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences |
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| Abstract: | Changfeng silver carp (Hypophthalmichthys molitrix) is a new silver carp bred by the Yangtze River Fisheries Research Institute of the Chinese Academy of Fishery Sciences. It has many advantages, such as a quick growth rate, a high body size, and a neat appearance, which are associated with more excellent growth characteristics than those of common H. molitrix. Up to the present, Changfeng H. molitrix has been spread in 26 provinces (cities and areas) in China, and exceptional economic and social benefits have been achieved. However, the study on H. molitrix at home and abroad mainly focuses on developing microsatellite markers, analyzing genetic structure, and establishing the genetic linkage map. Few molecular markers associated with H. molitrix growth traits have been thought of, and it is not known whether these markers can be used in the selection and breeding of Changfeng H. molitrix. This study aimed to screen out the molecular markers related to the growth traits of Changfeng H. molitrix to guide the germplasm identification and selective breeding. In this study, 15 polymorphic microsatellite markers were used to analyze the genetic diversity and growth trait association of Changfeng H. molitrix at 6, 17 and 36 months. The results show that the observed heterozygosity (Ho) and expected heterozygosity (He) of each month-old group changfeng H. molitrix were higher than 0.5. The average PIC values were 0.555, 0.445, and 0.490 respectively at each age; indicating that the population was highly genetically diverse. The results of associations between microsatellite markers and growth traits revealed that locus BL55 and BL109 were significantly associated with body height at 6-month-old (P<0.05); S65 was found Significantly associated with body height at 17-month-old (P<0.05); locus BL55 was significantly associated with total length (P<0.05), and BL106-2 and BL116 were significantly associated with body weight at 36-month-old (P<0.05). The different growth traits associated with different months of age of the same marker may be due to the different growth trends of Changfeng H. molitrix. Locus BL55 on chromosome 15 has the same allele containing DNA fragment length of 231 at different month ages, and it is preliminarily speculated that the genotypes 225/231, as well as 231/245, will have negative and positive effects, respectively. To further examine whether this locus can be used for molecular marker-assisted breeding in H. molitrix populations, two wild populations of H. molitrix from Yangtze River and Xiangjiang were selected for analysis. The results showed that the allele of the 231bp fragment existed in both the Yangtze River and Xiangjiang populations. This result preliminarily confirmed that the marker BL55 was determined as a candidate marker related to growth traits, which has a specific reference value for genetic improvement and selective breeding of H. molitrix. |
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| Keywords: | Changfeng silver carp microsatellite marker growth trait correlation analysis |
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