| 金属蛋白酶组织抑制剂在皱纹盘鲍抗弧菌免疫中的作用 |
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| 引用本文： | 张明辉,章骞,林锦锦,张凌晶,刘光明,陈玉磊,曹敏杰.金属蛋白酶组织抑制剂在皱纹盘鲍抗弧菌免疫中的作用[J].水产学报,2022,46(1):126-135. |
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| 作者姓名： | 张明辉 章骞 林锦锦 张凌晶 刘光明 陈玉磊 曹敏杰 |
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| 作者单位： | 集美大学海洋食品与生物工程学院,福建厦门361021;集美大学海洋食品与生物工程学院,福建厦门361021;水产品深加工技术国家地方联合工程研究中心,福建厦门361021 |
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| 基金项目： | 国家重点研发计划项目（2018YFD0901004）；国家自然科学基金项目（31702372）；国家贝类产业技术体系（CARS-49） |
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| 摘 要： | 由弧菌感染引起的疾病是影响鲍存活率的主要因素,实验以皱纹盘鲍为研究对象,探讨金属蛋白酶组织抑制剂(tissue inhibitor of metalloproteinase,TIMP)在皱纹盘鲍抗弧菌免疫中的功能及其与基质金属蛋白酶1 (matrix metalloproteinase 1,MMP-1)的相互作用关系....
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| 关 键 词： | 皱纹盘鲍 副溶血性弧菌 金属蛋白酶组织抑制剂 基质金属蛋白酶l 相互作用 先天性免疫 |
| 收稿时间： | 2020/10/4 0:00:00 |
| 修稿时间： | 2021/1/27 0:00:00 |
Role of tissue inhibitor of metalloproteinase in the anti-Vibrio immunity of Haliotis discus hannai |
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| ZHANG Minghui,ZHANG Qian,LIN Jinjin,ZHANG Lingjing,LIU Guangming,CHEN Yulei,CAO Minjie.Role of tissue inhibitor of metalloproteinase in the anti-Vibrio immunity of Haliotis discus hannai[J].Journal of Fisheries of China,2022,46(1):126-135. |
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| Authors: | ZHANG Minghui ZHANG Qian LIN Jinjin ZHANG Lingjing LIU Guangming CHEN Yulei CAO Minjie |
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| Institution: | Jimei University,Jimei University,Jimei University,Jimei University,Jimei University,Jimei University,Jimei University |
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| Abstract: | As an important economical shellfish in coastal area of China, abalone is susceptible to bacterial infection, especially Vibiro parahemolyticus (V. parahemolyticus). Similar to other invertebrates, Haliotis discus hannai resist pathogen infection to the host by innate immune response. Tissue inhibitors of metalloproteinases (TIMPs) are endogenous protein regulators of the matrix metalloproteinases (MMPs) family. As MMP-1 was engaged in the innate immunity of abalone, the involvement of TIMP in the regulation of MMP-1 expression and activity as well as the immune responses merits further investigation. In this study, the role of TIMP in the innate immune responses of H. discus hannai against V. parahaemolyticus infection and the interaction between TIMP and MMP-1 were investigated. The full length cDNA sequence of TIMP was obtained, and the expression of TIMP in different tissues of H. discus hannai were analyzed post Vibrio infection. The cloned TIMP cDNA sequence was 2291 bp in length. In the early stage of V. parahaemolyticus infection, the expression of TIMP in hemocytes and gill tissues were significantly up-regulated. To study the interaction between TIMP and MMP-1 in the anti-Vibrio immunity of abalone, RNA interference technology was used to knock down the expression levels of TIMP and MMP-1. After the silencing of MMP-1 gene in abalone, TIMP expression was significantly up-regulation. When TIMP expression was inhibited, MMP-1 expression decreased significantly. These results indicated that MMP-1 expression was positively regulated by TIMP, while TIMP expression was negatively regulated by MMP-1 in abalone. In conclusion, the present study established the interaction between TIMP and MMP-1 in abalone innate immunity against V. parahemolyticus infection. It also provided new insights for the prevention and control of pathogens in abalone culture. |
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| Keywords: | Haliotis discus hannai Vibrio parahaemolyticus Tissue inhibitor of metalloproteinase Matrix metalloproteinase 1 Interaction Innate immunity |
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