| 侵染紫松果菊的黄瓜花叶病毒分离物鉴定 |
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| 引用本文： | 李桂芬,朱水芳,周群,施宗伟,张永江,李明福.侵染紫松果菊的黄瓜花叶病毒分离物鉴定[J].植物保护,2007,33(1):54-56. |
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| 作者姓名： | 李桂芬 朱水芳 周群 施宗伟 张永江 李明福 |
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| 作者单位： | 中国检验检疫科学研究院动植物检验检疫研究所 北京100024(李桂芬，朱水芳，施宗伟，张永江，李明福)，山东省莱西市农业局植保站 266600(周群) |
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| 摘 要： | 从表现黄花叶症状的紫松果菊病株上获得分离物2-1-2,电镜下可见直径约30 nm的球状病毒粒体,其与黄瓜花叶病毒抗体呈强的阳性反应,ds-RNA的谱带类型与本实验室保存的标准黄瓜花叶病毒株系相同。通过生物学、病毒粒体观察、血清学以及病毒核酸双链试验结果,确定该病毒分离物为黄瓜花叶病毒。
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| 关 键 词： | 紫松果菊 黄瓜花叶病毒 鉴定 |
| 修稿时间： | 2006-03-212006-06-20 |
Identification of Cucumber mosaic virus isolated from Echinacea purpurea |
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| Li Guifen;Zhu Shuifang;Zhou Qun;Shi Zongwei;Zhang Yongjiang;Li Mingfu.Identification of Cucumber mosaic virus isolated from Echinacea purpurea[J].Plant Protection,2007,33(1):54-56. |
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| Authors: | Li Guifen;Zhu Shuifang;Zhou Qun;Shi Zongwei;Zhang Yongjiang;Li Mingfu |
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| Institution: | 1. Institute of Animal and Plant Quarantine, Chinese Academy of Inspection & Quarantine, Beijing100024, China;2. Laixi Station of Plant Protection of Agricultural Bureau, Shandong266600, China |
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| Abstract: | The virus particles of about 30nm in diameter were isolated from Echinacea purpurea Moench showing systemic sympotoms,including yellow mosaic on leaves.The virus-infected plants reacted positively with the Cucumber mosaic virus(CMV) antibody in triple antibody sandwich enzyme-linked immunosorbent assay(TAS-ELISA).The type of the double-stranded RNA pattern of the isolate was the same as the standard CMV.On the basis of the biology,particle morphology,serology and ds-RNA pattern of the virus,it was concluded that the virus isolated from E.purpurea in Huairou,Beijing is Cucumber mosaic virus. |
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| Keywords: | Echinacea purpurea Moench Cucumber mosaic virus identification |
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