Application of ISSRs for cultivar identification and assessment of genetic relationships in rose |
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| Authors: | L Crespel A Pernet M Le Bris S Gudin and L Hibrand Saint Oyant |
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| Institution: | Meilland International, Domaine Saint André, F-83340 Le Cannet des Maures, France, E-mail:;;INRA, UMR 1259, Génétique et Horticulture, F-49071 Beaucouzé, France;;Institut Méditerranéen d'Ecologie et de Paléoécologie (IMEP), UniversitéPaul Cézanne (Aix-Marseille III), Facultéde Saint Jérôme, Service 442, Av. Escadrille Normandie-Niemen, F-13397 Marseille, Cedex 20, France;;Present address: INH, UMR 1259, Génétique et Horticulture, 2, rue Le Nôtre, F-49045 Angers, Cedex 1, France |
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| Abstract: | The inter-simple sequence repeat (ISSR) technique was evaluated for its applicability to cultivar identification and assessment of genetic relationships in rose. Nine ISSR primers that revealed informative patterns were selected to fingerprint (by Resophor agarose gel electrophoresis) 33 cultivars, including unrelated cultivars, sports and offspring obtained by sexual propagation from the same initial variety. A total of 159 fragments were generated using these nine primers, 149 of which (93.7%) were polymorphic. All 33 rose cultivars, except for the known colour sports, were differentiated. A Jaccard's dissimilarity coefficient matrix of the cultivars showed a low dissimilarity level between them (mean dissimilarity: 0.45). An unweighted pair group method with the arithmetic averages dendrogram and principal coordinates analysis separated them into four main groups according to their horticultural classification and pedigree. The ISSR technique is therefore a potentially useful tool for cultivar identification and assessing genetic relationships in rose because it is simple, fast, cost-effective, reliable and highly discriminating. |
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| Keywords: | Rosa hybrida L cultivar sport inter-simple sequence repeats polymorphism |
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