| Bt转基因抗虫粳稻品种选育中若干问题研究 |
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| 引用本文： | 沈圣泉,吴殿星,崔海瑞,夏英武,舒庆尧.Bt转基因抗虫粳稻品种选育中若干问题研究[J].浙江大学学报(农业与生命科学版),2003,29(5):499-503. |
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| 作者姓名： | 沈圣泉 吴殿星 崔海瑞 夏英武 舒庆尧 |
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| 作者单位： | 浙江大学,原子核农业科学研究所,浙江,杭州,310029 |
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| 基金项目： | 美国洛氏基金;国家转基因专项;浙江省重点项目资助. |
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| 摘 要： | 以Bt转基因水稻"克螟稻"为供体,与农艺性状优良的粳稻品种杂交,对杂交后代抗虫品种选育中GUS组织染色辅助选择、农艺性状选择对Bt基因纯合影响、混合选择与外源基因传递、及自然抗虫鉴定选择效果等方面作了研究,结果发现采用系谱法并结合GUS组织染色辅助选择的Bt转基因粳稻育种效果为最佳.经8代系谱选择,育成了3 个集高产和抗虫于一体的优良粳稻新品系.
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| 关 键 词： | 水稻 Bt 转基因 杂交育种 抗虫性 |
| 文章编号： | 1008-9209(2003)05-0499-05 |
| 修稿时间： | 2002年6月24日 |
Study on some problems in breeding insect-resistant Bt transgenic Japonica rice |
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| SHEN Sheng-quan,WU Dian-xing,CUI Hai-rui,XIA Ying-wu,SHU Qing-yao.Study on some problems in breeding insect-resistant Bt transgenic Japonica rice[J].Journal of Zhejiang University(Agriculture & Life Sciences),2003,29(5):499-503. |
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| Authors: | SHEN Sheng-quan WU Dian-xing CUI Hai-rui XIA Ying-wu SHU Qing-yao |
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| Abstract: | Using the Bt rice "KMD" as the insect-resistant donor, three transgenic Japonica rice lines with high yield and insect resistance were successfully developed from crosses between the Bt rice and Japonica rice variety after pedigree selections lasting eight generations through GUS tissue staining-aid-ed selection as well as laboratory and field insect-resistant evaluation. This fact indicates that the poor agronomic traits in the original transgenic rice plants can be improved through the conventional crossing breeding techniques. The effects of four kinds of breeding techniques, which involved screening the insect-resistant transgenic rice plants as revealed by GUS tissue staining, agronomic traits selection, bulk selection, field insect-resistance selection, were comparatively studied. The result showed that it is desirable to create insect-resistant Bt transgenic new Japonica rice via integral utilization of pedigree method and GUS tissue staining-aided selection. |
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| Keywords: | rice i Bt gene transgene crossing breeding insect resistance |
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