| 亚麻CAD基因克隆及序列分析 |
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| 引用本文： | 黄海燕,王玉富,薛召东,邱财生,郝冬梅.亚麻CAD基因克隆及序列分析[J].湖北农业科学,2008,47(5):496-498. |
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| 作者姓名： | 黄海燕 王玉富 薛召东 邱财生 郝冬梅 |
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| 作者单位： | 中国农业科学院麻类研究所,长沙,410205 |
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| 基金项目： | 国家科技支撑计划
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农业部行业计划项目
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国家引进国际先进农业科学技术计划(948计划) |
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| 摘 要： | 以亚麻(Linum usitatissimum L.)为材料,研究木质素代谢的关键酶亚麻肉桂醇脱氢酶基因序列.分离了高纯度的RNA,以RNA为模板,用简并引物以RT-PCR的方法,克隆了亚麻CAD基因cDNA部分序列,长度为477bp,编码159个氨基酸残基.此cDNA序列为亚麻CAD基因序列.
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| 关 键 词： | 亚麻 CAD基因 基因克隆 序列分析 |
Clone of Partial Sequence of Cinnamyl Alcohol Dehydrogenase Gene in Flax |
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| HUANG Hai-yan,WANG Yu-fu,XUE Zhao-dong,QIU Cai-sheng,HAO Dong-mei.Clone of Partial Sequence of Cinnamyl Alcohol Dehydrogenase Gene in Flax[J].Hubei Agricultural Sciences,2008,47(5):496-498. |
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| Authors: | HUANG Hai-yan WANG Yu-fu XUE Zhao-dong QIU Cai-sheng HAO Dong-mei |
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| Institution: | HUANG Hai-yan,WANG Yu-fu,XUE Zhao-dong,QIU Cai-sheng,HAO Dong-mei(Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciencrs,Changsha 410205,China) |
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| Abstract: | The object of this study was to examine the sequence of flax CAD and investigate flax lignin biosynthesis pathway.High quality RNA was extracted from flax(Linum usitatissimum L.)and used as a template for cloning the cinnamyl alcohol dehydrogenase gene cDNA by RT-PCR with degenerate primers under gradient temperature.Colonies with target foreign DNA inserts was identified by PCR.Recombinant pGEM-T plasmid was identified by restriction digestion and PCR,also sequence analysis.Flax cinnamyl alcohol dehydrogen... |
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| Keywords: | Linum usitatissimum CAD gene gene cloning sequence analysis |
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