| 喷施Bt杀虫剂对土壤微生物生物量和多样性的影响 |
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| 引用本文： | 陈梦扬,魏健,葛高波,孙铭,冯秀智,吴可人,李永春,徐秋芳.喷施Bt杀虫剂对土壤微生物生物量和多样性的影响[J].浙江农林大学学报,2013,30(5):662-668. |
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| 作者姓名： | 陈梦扬 魏健 葛高波 孙铭 冯秀智 吴可人 李永春 徐秋芳 |
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| 作者单位： | 1.浙江农林大学 环境与资源学院，浙江 临安 311300;2.浙江农林大学 浙江省森林生态系统碳循环与固碳减排重点实验室，浙江 临安 311300 |
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| 基金项目： | 国家自然科学基金资助项目（31200473）;浙江农林大学人才启动基金资助项目（2012FR10） |
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| 摘 要： | 应用氯仿熏蒸法和聚合酶链式反应?鄄变性梯度凝胶电泳（PCR-DGGE）技术研究了菜地土和松林土喷施苏云金杆菌Bacillus thuringiensis（Bt）杀虫剂后，土壤微生物量和细菌群落结构及多样性的变化特征。研究结果表明:菜地土喷施Bt杀虫剂后，土壤微生物量呈现先减少后增加再减少的变化趋势；松林土喷施Bt杀虫剂后，土壤微生物量碳则呈现出增加的趋势；2种土壤均在喷施1 315 ghm－2的Bt杀虫剂后，微生物量碳达到最大值。微生物群落结构分析表明，菜地与松林土壤变性梯度凝胶电泳（DGGE）图谱带型有较大的差异，菜地土壤细菌群落具有更高的丰富度。土壤类型是决定微生物群落结构相似性的关键因素，Bt杀虫剂梯度喷施并不足以形成新的群落结构。菜地土喷施Bt杀虫剂后微生物多样性指数显著上升（P＜0.05），而松林土则表现出显著下降（P＜0.05）或不变的趋势。图3表3参20
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| 关 键 词： | 土壤学 苏云金杆菌（Bt）杀虫剂 土壤微生物量 细菌群落结构 多样性指数 |
| 收稿时间： | 2012-12-21 |
Soil microbial biomass and biotic diversity with Bt insecticide |
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| CHEN Mengyang,WEI Jian,GE Gaobo,SUN Ming,FENG Xiuzhi,WU Keren,LI Yongchun,XU Qiufang.Soil microbial biomass and biotic diversity with Bt insecticide[J].Journal of Zhejiang A&F University,2013,30(5):662-668. |
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| Authors: | CHEN Mengyang WEI Jian GE Gaobo SUN Ming FENG Xiuzhi WU Keren LI Yongchun XU Qiufang |
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| Institution: | 1.School of Environmental and Resources Science，Zhejiang A & F University，Lin’an 311300，Zhejiang，China;2.Zhejiang Provincial Key Laboratory of Carbon Cycling in Forest Ecosystems and Carbon Sequestration, Zhejiang A & F University，Lin’an 311300，Zhejiang，China |
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| Abstract: | Environmental safety assessment of soil microbial is essential for development and application of Bt insecticide. To analyze the effects of Bt insecticide on soil microbial in vegetable and pine forest soils，a chloroform fumigation-extraction method and a denaturing gradient gel electrophoresis（DGGE）were used to assess microbial biomass and the bacterial community after spraying insecticide Bt with three concentration gradients. Results indicated that, after spraying Bt insecticide at 375，1 315，and 2 255 ghm－2，soil microbial biomass carbon（MBC）for vegetable soil, significantly decreased, increased and then decreased（P＜ 0.05）， respectively；whereas, for a pine forest soil, when spraying at concentration gradients of 1 315，2 255 ghm－2，MBC significantly increased（P＜0.05）. Compared with the samples without spraying Bt，MBC for both vegetable soils and pine forest soils reached a maximum at 1 315 ghm－2 of Bt. Also, compared to pine forest soils，DGGE patterns for vegetable soils revealed a higher richness in the bacterial community. After spraying Bt insecticide on vegetable soils，soil bacterial community diversity indices significantly increased（P ＜0.05），but for pine forest soils they significantly decreased（P＜0.05）or were unchanged with different concentration gradient. In this study，soil types were a key determinant of soil bacterial community structure, and spraying Bt insecticide did not lead to new community structures.［Ch，3 fig. 3 tab. 20 ref.］ |
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