| 应用A蛋白免疫电镜技术快速检测病株中大麦黄花叶病毒 |
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| 引用本文： | 陈剑平,徐均焕.应用A蛋白免疫电镜技术快速检测病株中大麦黄花叶病毒[J].植物病理学报,1991,21(1):73-77. |
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| 作者姓名： | 陈剑平 徐均焕 |
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| 作者单位： | 1 浙江省农业科学院病毒实验室;2 浙江农业大学电子显微镜室 |
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| 基金项目： | 农业部“七五”重点课题 |
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| 摘 要： | 用A蛋白免疫电镜技术,检测大麦病汁液中的大麦黄花叶病毒(BaYMV),其捕获抗血清的适宜工作浓度范围很宽,320-20,480倍均能达到满意的结果;抗原孵育条件以室温(约25℃)下30分钟或冰箱(约4℃)中3小时,捕获到的病毒粒子数量较多。应用这项技术,在稀释3,125倍的病汁液中还能检测到BaYMV粒子,比普通免疫电镜方法的灵敏度至少高5倍。BaYMV在病株中的分布以症状明显的花叶中含量最高,黄化叶中较少,茎和根中找不到病毒。利用该技术可以快速而灵敏地进行大麦品种抗病性的鉴定与筛选。本文最后还讨论了BaYMV的稳定性。
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| 关 键 词： | 大麦 黄花叶病毒 BaYMV 检测 |
RAPID DETECTION ON BARLEY YELLOW MOSAIC VIRUS (BaYMV) BY PROTEIN A IMMUNOSORBENT ELECTRON MICROSCOPY |
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| Chen Jianping,Ruan Yili,Xu Jiunhuan,Hong Jian.RAPID DETECTION ON BARLEY YELLOW MOSAIC VIRUS (BaYMV) BY PROTEIN A IMMUNOSORBENT ELECTRON MICROSCOPY[J].Acta Phytopathologica Sinica,1991,21(1):73-77. |
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| Authors: | Chen Jianping Ruan Yili Xu Jiunhuan Hong Jian |
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| Institution: | 1 Virology Laboratory, Zhejiang Academy of Agricultural Sciences, Hangzhou;2 Electron Microscopy Laboratory, Zhejiang Agriculture University, Hangzhou |
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| Abstract: | Barley yellow mosaic virus (BaYMV) was rapidly detected by protein A immunosorbent electron microscopy in barley plants suspected to be infected with this virus. Wide range of homogenous antiserum dilution (320-20,480) in 0.03 M phosphate buffer pH 7.0 for virus particle trapping and incubation for 30min. at room temperature (25℃) or 3 hrs at refrigerator (4℃) could give satisfactory results. Attachment of virus particles from infected sap diluted 3, 125 times to protein A-antiserumcoated electron microscopy grids provided a test that was at least five times more sensitive than standard immunosorbent electron microscopy technique for BaYMV detection. BaYMV particles mainly distributed in top mosaic leave and no one checked in stems and roots. |
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