| 激素诱导下不同培养方式对马铃薯微型薯的诱导效应 |
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| 引用本文： | 彭晓莉,王蒂,张金文,吴旺泽.激素诱导下不同培养方式对马铃薯微型薯的诱导效应[J].甘肃农业大学学报,2006,41(1):16-19. |
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| 作者姓名： | 彭晓莉 王蒂 张金文 吴旺泽 |
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| 作者单位： | 甘肃农业大学农学院,甘肃,兰州,730070;甘肃省作物遗传改良与种质创新重点试验室,甘肃,兰州,730070 |
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| 基金项目： | 国家高技术研究发展计划“863”项目(编号:2001AA241131) |
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| 摘 要： | 以两个马铃薯普通栽培种“Atlantic”和“甘农薯2号”的无毒试管苗为材料,研究了离体条件下,GA3和IAA对匍匐茎发生和结薯的影响,及在此条件下固体培养、固液双层培养和液体培养3种不同培养方式对马铃薯微型薯诱导和发育的影响.试验结果表明:GA3和IAA在诱导匍匐茎发生和增加单株结薯数上有重要作用,尤以液体培养条件下的效果最为明显;激素诱导下3种培养方式对微型薯的诱导效率在单瓶薯数、单瓶薯重、薯块平均直径及结薯率等方面差异极显著,在单薯重上差异显著,其诱导效应依次为:液体培养>固液双层培养>固体培养.
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| 关 键 词： | 马铃薯 微型薯 固体培养 固液双层培养 液体培养 激素 |
| 文章编号： | 1003-4315（2006）01-0016-04 |
| 收稿时间： | 2005-10-20 |
| 修稿时间： | 2005-10-20 |
Induction effects of different culture ways on potato microtubers under hormone treatments |
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| PENG Xiao-li,WANG Di,ZHANG Jin-wen,WU Wang-ze.Induction effects of different culture ways on potato microtubers under hormone treatments[J].Journal of Gansu Agricultural University,2006,41(1):16-19. |
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| Authors: | PENG Xiao-li WANG Di ZHANG Jin-wen WU Wang-ze |
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| Institution: | 1. College of Agronomy, Gansu Agricultural University,Lanzhou 730070 ;2. Gansu Key Laboratory of Crop Genetic and Germplasm Enhaneement,Lanzhou 730070,China |
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| Abstract: | Virus-free planlets of potato(Solanum tubersum L.)cultivars'Atlantic'and 'Gannongshu No.2' were employed in the experiment.The effects of GA_(3)and IAA on the formation of stolons and produltion of microtubers,and the effects of solid culture,double layer culture and liquid culture on induction and development of potato microtubers under in vitro condition were investigated.The results indicated that GA_(3)and IAA had significant influence on the stolons formation and producing more microtubers per plantlet,especially in liquid culture.For the number,the weight and the average diameter of microtubers per vessel,there existed significant differences among three culture ways,and it had difference for the weight of single microtuber.The liquid culture was the best to produce the potato microtubers. |
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| Keywords: | potato microtuber solid culture double layer culture liquid culture hormone |
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