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UV-B辐射下离体芒果成年叶抗氧化响应
引用本文:刘鹏,周开兵,丁姗,蔡惠珍.UV-B辐射下离体芒果成年叶抗氧化响应[J].贵州大学学报(农业与生物科学版),2010,29(5):397-402.
作者姓名:刘鹏  周开兵  丁姗  蔡惠珍
作者单位:海南大学热带作物种质资源保护与开发利用教育部重点实验室;
基金项目:海南省自然科学基金项目
摘    要:利用分光光度法,研究了增强UV-B辐射条件下"台农一号"芒果盆栽苗离体成年叶片抗氧化响应。结果表明:UV-B处理使叶片丙二醛含量和相对电导率升高,叶绿素含量和叶绿素a/b降低,说明处理引起了叶片损伤,且随处理时间的延长叶片伤害加重;UV-B处理的叶片可溶性蛋白、类黄酮、类胡萝卜素含量和过氧化氢酶活性显著高于对照,Vc和还原型谷胱甘肽含量明显低于对照,超氧化物歧化酶活性表现为前期(8 h前)高于对照和后期(10 h后)低于对照,说明增强UV-B辐射可诱导叶片细胞通过清除活性氧、积累保护色素直接吸收部分UV-B辐射和增强渗透调节能力来提高抗增强UV-B辐射损伤的能力。

关 键 词:分光光度法  增强UV-B辐射  芒果  成年叶  损伤  保护反应

Antioxidative Response of Adult Mango (Mangifera indica L.) Leaves to Enhanced UV-radiation
LIU Peng,ZHOU Kai-bing,DING Shan,CAI Hui-zhen.Antioxidative Response of Adult Mango (Mangifera indica L.) Leaves to Enhanced UV-radiation[J].Journal of Mountain Agriculture & Biology,2010,29(5):397-402.
Authors:LIU Peng  ZHOU Kai-bing  DING Shan  CAI Hui-zhen
Institution:LIU Peng,ZHOU Kai-bing,DING Shan,CAI Hui-zhen(Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources of Education Ministry,Hainan University,Haikou Hai'nan 570228,China)
Abstract:Adult leaves of potted mango young trees were used to investigation the response in the antioxidation to the enhanced UV-B radiation by infrared absorption spectrometry in the present work.The results showed that after UV-B treatment the content of MDA and the relative conductivity were elevated,while the content of chlorophyll and the ratio of chlorophyll a/b decreased.It indicated the enhanced UV-B radiation caused the damages of leaves,which would be worse with progression of stress.The UV-B treated leav...
Keywords:Spectrophotometry  enhanced UV-B radiation  mango  adult leaf  damage  antioxidative response  
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