| 氮素转化菌对茶树根际土壤微生物群落和养分含量的影响 |
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| 引用本文： | 韩晓阳,张丽霞,黄晓琴,董玉惠,李智,尚涛.氮素转化菌对茶树根际土壤微生物群落和养分含量的影响[J].茶叶科学,2015(5):405-414. |
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| 作者姓名： | 韩晓阳 张丽霞 黄晓琴 董玉惠 李智 尚涛 |
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| 作者单位： | 1. 山东农业大学园艺科学与工程学院，山东 泰安 271018; 作物生物学国家重点实验室，山东 泰安 271018;2. 泰安市农业局,山东 泰安,271000 |
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| 基金项目： | “十二五”国家科技支撑计划，泰安生态茶园关键技术集成研究及产业化示范，山东农业大学青年科技创新基金 |
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| 摘 要： | 为了研究氮素转化菌对茶树根际土壤微生态环境的影响,本试验以山东泰安2年生茶树根际土壤为研究对象,设置枯草芽孢杆菌(T1)和褐球固氮菌(T2)两个接种处理,以不接种为对照(CK)。采用培养计数法与末端限制性片段长度多态性技术(T-RFLP),测定不同时期根际土壤中细菌、真菌、放线菌、氨化细菌、固氮菌、硝化细菌的数量及接种后微生物多样性的变化。同时测定接种后土壤中养分含量的变化。结果表明,接种60 d后T1和T2处理细菌数量显著高于CK,但对真菌及放线菌的影响不显著。整个试验过程氨化细菌和固氮菌数量高于CK,氨化细菌以T1处理最多,固氮菌以T2处理最多,硝化细菌数量始终是CK显著高于T1和T2处理。基于T-RFLP图谱分析,与对照组相比,菌剂组均增加了土壤微生物多样性和丰富度,各物种在数量上的均匀程度变大。在土壤养分方面,T1、T2菌剂处理组铵态氮浓度显著高于对照组,硝态氮含量低于对照。在速效P方面,T1、T2含量比CK分别显著提高了8%、25%。在速效K方面,T1、T2含量显著高于CK,提高了15%和11%。枯草芽孢杆菌和褐球固氮菌菌剂改善了茶树根际土壤微生态环境,提高了土壤养分,表现出正面效应,是一种生态安全性较高的生物菌剂。
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| 关 键 词： | 固氮菌 氨化细菌 T-RFLP 微生物多样性 土壤养分 |
Effect of Nitrogen Transformation Bacteriaon Microbial Communityand Nutrient Contentsin Rhizosphere Soil of Tea Plant |
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| Abstract: | The effects of nitrogentransformationbacteriaonmicro-ecological environmentof the rhizosphere soil of 2 years old tea plant were studied in Taian City of Shandong Province. TheBacillus subtilis(T1) andAzotobacter chroococcum(T2) were set up as inoculation treatments, and thenoninoculationasthe controlin this research.The number of bacteria, fungi, actinomycetes, ammonifying bacteria, azotobacter, nitrifying bacteria and the modification of microbial diversity in rhizosphere soil in different periods weremeasured, using themethodsofculture counting and T-RFLP. Meanwhile, the nutrient contentsin the soil were also determined. The results showed that the bacterial number of T1 and T2 were significantly higher than CK, but there were no significant impact on fungiand actinomycetes. In thewhole test process, the number of ammonifying bacteria andazotobacterwere higher thanthat ofCK, among which ammonifying bacteria of T1 was the most, azotobacter of T2 was the most; the nitrifying bacteria numbersof CK was significantly higher than T1and T2. Based on T-RFLP mapping analysis and compared with the control group, microbial diversity,therichness and evennesswere rise inthe bacterium treatments. The ammonium nitrogen concentration of T1 and T2 were significantly higher thanthat ofCK,but the nitrate concentration were lower thanthat ofCK. The available P concentration of T1 and T2 significantly increased by 8%and 25% for CK. Meanwhile, the available K concentration of T1 and T2 significantly increasedby 15% and 11% for CK.The inoculationofBacillus subtilisandAzotobacter chroococcumshowed the positive effects, which could improve micro-ecological environment and soil nutrientsof tea rhizospheric soil. |
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| Keywords: | nitrogen fixing bacteria ammonifying bacteria T-RFLP microbialdiversity soil nutrient |
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