| 柑橘花期蓟马的种类与鉴定 |
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| 引用本文： | 阙海勇,黄丽莉,薛芳森,车飞,何海敏,陈超.柑橘花期蓟马的种类与鉴定[J].江西植保,2013(4):355-358. |
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| 作者姓名： | 阙海勇 黄丽莉 薛芳森 车飞 何海敏 陈超 |
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| 作者单位： | [1]江西出入境检验检疫局检验检疫综合技术中心,江西南昌330038 [2]江西农业大学昆虫研究所,江西南昌330045 |
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| 基金项目： | 国家质检总局科技计划项目（2012IK279） |
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| 摘 要： | 为明确柑橘花期蓟马种类，以柑橘园为实验基地，系统调查橘园中蓟马的种类。经形态学鉴定共发现3种蓟马，且3种蓟马的形态分别与棕榈蓟马（Thrips palmi Karny）、淡色蓟马（Thrips flavus Schrank）和华简管蓟马（Haplothris chinesis Pfiesner）极其相似。对3种蓟马分别进行单头虫体的基因组DNA提取，对其ITS2基因序列进行PCR扩增并测序，测序结果登入NCBI数据进行比对，结果表明3种蓟马分别与棕榈蓟马（Thrips palmi Kamy）、淡色蓟马（Thrips flavus Schrank）和华简管蓟马（Haplothris chinesis Priesner）的相似度均达99％。
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| 关 键 词： | 柑橘花期 蓟马种类 鉴定 |
Identification of Citrus Thrips at Stage of Flowering |
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| QUE Hai-yong,HUANG Li-li,.,XUE Fang-sen,CHE Fei,HE Hai-min,CHEN Chao.Identification of Citrus Thrips at Stage of Flowering[J].Jiangxi Plant Protection,2013(4):355-358. |
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| Authors: | QUE Hai-yong HUANG Li-li XUE Fang-sen CHE Fei HE Hai-min CHEN Chao |
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| Institution: | 1 .Comprehensive Centre of Inspection and Quarantine Technology, Entry-Exit Inspection and Quarantine Bureau of Jiangxi, Nanchang 330038, China; 2. Institute of Entomology, Jiangxi Agricultural University, Nanchang 330045, China) |
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| Abstract: | In order to understand the abundance of thrips species in blossoming citrus, we investigated the species ofthrips in citrus fields in Anyuan County, Xinfeng County and Nanchang suburbs from March to April in 2012. There were three specieses ofthrips by morphology identification, which were much similar to Thripspalmi Karny, Thripsflavus Schrank and Haplothris chinesis Priesner. The single head insect body genomic DNA of these three kinds of thrips were extracted, and then the ITS2 gene sequences were amplified by PCR and were sequenced respectively. The sequencing results showed that the average similarities of these three kinds of thrips were up to 99% with Thrips palmi Kamy, Thrips flavus Schrank and Haplothris chinesis Priesner contrasted by NCBI data. |
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| Keywords: | citrus thrips species identification |
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