| 60Co-γ射线对菊花组培苗的诱变效应 |
| |
| 引用本文： | 王晶,刘录祥,ZHAO Shi-rong,赵世荣,郭会君,赵林姝,陈文华.60Co-γ射线对菊花组培苗的诱变效应[J].农业生物技术学报,2006,14(2):241-244. |
| |
| 作者姓名： | 王晶 刘录祥 ZHAO Shi-rong 赵世荣 郭会君 赵林姝 陈文华 |
| |
| 作者单位： | 中国农业科学院作物科学研究所,农业部农业核技术与航天育种重点开放实验室,北京,100081 |
| |
| 摘 要： | 对菊花(Chrysanthemum morifolium Ramat.)品种秋之山的组培苗, 采用5～30 Gy的60Co-γ射线照射处理。研究了γ射线对组培苗生长、增殖和生根的影响以及对组培再生植株移栽后生长开花的影响。结果表明20 Gy是γ射线处理菊花组培苗的致死剂量；从生根和继代培养生长和增殖的结果看，诱变处理的最佳剂量在10 Gy左右；组培再生植株移栽后发现，10 Gy处理的植株中花色变异率为8.2%。与对照花鲜艳的纯黄色相比，变异花增加了不同深浅的红色素，且突变植株均为同质稳定突变。
|
| 关 键 词： | Co-γ射线 辐射诱变 菊花 组织培养 |
| 文章编号： | 1006-1304（2006）02-0241-04 |
| 收稿时间： | 2005-4-5 |
| 修稿时间： | 2005-04-052005-05-23 |
Effect of 60Co-γ Rays on In vitro Mutagenesis of Chrysanthemum morifolium Ramat |
| |
| ZHAO Shi-rong.Effect of 60Co-γ Rays on In vitro Mutagenesis of Chrysanthemum morifolium Ramat[J].Journal of Agricultural Biotechnology,2006,14(2):241-244. |
| |
| Authors: | ZHAO Shi-rong |
| |
| Abstract: | The microshoots of in vitro-cultured Chrysanthemum morifolium cv. Qiuzhishan were irradiated with 5~30 Gy 60Co-γ rays. After irradiation the microcuttings were transferred to root-inducing medium and subculture medium respectively to study the inductive effects of γ rays on rooting, shoot growth and multiplication on media. The plant growth and flower characters were investigated after the regenerated plantlets were transplanted out of the protected aseptic environment. The results showed that 20 Gy was the lethal dose of 60Co-γ rays to irradiate the microshoots of in vitro -cultured Chrysanthemum morifolium cv. Qiuzhishan, while 10 Gy was the optimum dose to induce mutations. After bloom, 8.2% of the plants in the treatment of 10 Gy were selected for their flower color mutations. In contrast with the bright yellow flowers of the control, different shades of red color were observed in those mutant flowers. All the mutant plants were homogeneous and stable mutants. |
| |
| Keywords: | 60Co-gamma rays radiation induction Chrysanthemum morifolium in vitro culture |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《农业生物技术学报》浏览原始摘要信息 |
